A study of inner ear immune response after acoustic trauma

声学损伤通过细胞因子的表达和循环白细胞的浸润激活内耳免疫反应，从而增强内耳对抗原的反应。我使小鼠对一种抗原敏感，通过圆窗膜（RWN）将其注入耳蜗内，随后将小鼠暴露在噪音中。免疫组化分析表明，耳蜗内抗原激发，耳蜗内有炎性细胞浸润。然而，噪音暴露不能激活耳蜗免疫反应，这在抗原的存在。我推测较低声压级的纯音比倍频带噪声刺激引起的内耳免疫反应更低。

The aim of this study is to solve whether acoustic trauma, which activates an inner ear immune response with cytokine expression and infiltration of circulating leukocytes could augment the response to antigen. I sensitized mice to an antigen, injected it intracochlearly, through the round window membrane(RWN), and subsequently exposed the mice to noise.The immunohistochemical analysis indicates that infiltration of inflammatory cells was present in cochlea with intracochlear antigen challenge. However, noise exposure cannot activate a cochlear immune response, which in the presence of antigen. I speculate that pure tone with lower sound pressure level causes lower inner ear immune response than octave-band noise stimulation.