Haemophilus influenza Boifilm in Intractable Otitis Media

在生物膜状态下，流感嗜血杆菌对抗菌药物的敏感性远低于强直状态下的敏感性。我们清楚地显示了从急性中耳炎（AOM）儿童中分离的临床非典型流感嗜血杆菌（NTHi）的生物膜形成。目前的研究结果表明，NTHi生物膜的生产将与AOM的临床结果。抗菌剂暴露后的最小生物膜根除浓度测定和结晶紫测定表明，较高浓度的某种抗菌剂对生物膜有效。MBEC测定方法的建立将有助于合理筛选对微生物生物膜有效的抗菌药物和筛选新的有效抗菌化合物。

In the biofilm state, H.influenzae is much less susceptible to antimicrobial agents compared to the susceptibility in the planktonic state. We clearly showed the biofilm formation of clinical nontypable H.influenza (NTHi) isolates from acute otitis media(AOM) children. The current results suggest that production of biofilm by NTHi would be associated with clinical outcome of AOM. The minimal biofilm eradication concentration assay and crystal violet assay after antimicrobial exposure demonstrated that higher concentration of a certain antimicrobial agent to be effective for biofilms. The MBEC assay would be helpful for the rational selection of antimicrobial agents effective against microbial biofilms and for the screening of new effective antimicrobial compounds.