Haemophilus influenza Boifilm in Intractable Otitis Media

在生物膜状态下，与浮游状态下的敏感性相比，流感嗜血杆菌对抗菌剂的敏感性要低得多。我们清楚地显示了急性中耳炎（AOM）儿童的临床非分型流感嗜血杆菌（NTHi）分离物的生物膜形成。目前的结果表明，NTHi产生的生物膜可能与AOM的临床结果有关。抗菌药物暴露后的最小生物膜根除浓度试验和结晶紫试验表明，较高浓度的抗菌药物对生物膜有效。MBEC试验将有助于合理选择对微生物生物膜有效的抗菌药物和筛选新的有效抗菌化合物。

In the biofilm state, H.influenzae is much less susceptible to antimicrobial agents compared to the susceptibility in the planktonic state. We clearly showed the biofilm formation of clinical nontypable H.influenza (NTHi) isolates from acute otitis media(AOM) children. The current results suggest that production of biofilm by NTHi would be associated with clinical outcome of AOM. The minimal biofilm eradication concentration assay and crystal violet assay after antimicrobial exposure demonstrated that higher concentration of a certain antimicrobial agent to be effective for biofilms. The MBEC assay would be helpful for the rational selection of antimicrobial agents effective against microbial biofilms and for the screening of new effective antimicrobial compounds.