Cryopreservation and dried preservation at room temperature of human skin by vitrification procedure.

通过玻璃化程序对人体皮肤进行冷冻保存和室温干燥保存。

• 批准号: 21791745
• 负责人: TOKUYAMA Eijiro
• 金额: $ 2.41万
• 依托单位: Okayama University (2009, 2011)National Research Institute for Child Health and Development (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791745/
• 关键词: 皮膚移植; スキンバンク; クリプトビオシス

Cryopreservation of biological tissue needs special facilities. Vitrification procedure may solve this problem. It is known that vitrification procedure increases cryopreserved cell survival rate. We carried out cryopreservation and dried preservation at room temperature of human and rat skin by vitrification procedure. Besides, we carried out rat skin grafting. We could confirm cell structure maintain by transmission electron microscopy, but cells could not survive.

生物组织的超低温保存需要特殊的设备。玻璃化冷冻可以解决这一问题。众所周知，玻璃化冷冻可以提高冷冻保存细胞的存活率。采用玻璃化法对人和大鼠皮肤进行了超低温保存和室温干燥保存。此外，我们还进行了大鼠皮肤移植。透射电子显微镜可以证实细胞结构保持不变，但细胞不能存活。