Identification of tumor antigens in squamous cell carcinoma of head and neck by SEREX to determin immunogenically ideal targets for patients.

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SEREX-defined CCDC62-2 is a CT antigen that is immunogenic in a variety of cancer patients. We showed that CCDC62-2 is immunogenic in squamous cell carcinoma of head and neck (SCCHN) patients and its potential target for diagnostic reagent for SCCHN. To analyze CCDC62-2 protein expression in SCCHN, we produced a CCDC62-2 mAb. Immunohistochemical staining using a CCDC62-2 mAb showed that positive staining was observed heterogeneously in nuclei or cytoplasm of tumor cells. Humoral immune response of another SEREX-defined antigen, GKAP1, to a variety of cancer patients including SCCHN was examined. SCCHN patients produced antibody to GKAP1 protein, showing an additional candidate of the tumor marker for SCCHN.