Toward the development of biomimetic under-water adhesives inspired by barnacle larval cement

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Toward the development of biomimetic under-water adhesives inspired by larval cement of the barnacle, Megabalanus rosa, we have obtained the following results.1) We have developed a method to measure macroscopic forces for marine adhesives using an atomic force microscope. 2) We have developed a microassay to measure the interaction between proteins and clay nanosheets in mock-marine environments using Roche glass capillaries. 3) We have prepared two recombinant cement proteins ; rec-Ccg-57k and rec-Ccg-ASP, both of which, however, did not show positive effects on 1) and 2). 4) We found the strong antimicrobial activity of rec-Ccg-57k against marine GRAM-negative bacteria, Planococcus citreus. 5) In the course of searching unidentified cement components using next generation sequencing, we have found a gene homologous to M. rosa adult cement protein gene mrcp-100k.