Development of a novel estimation method of cell functions by creating non-adhesive single cell culture technology

制备了由聚乙二醇和精氨酸-甘氨酸-天冬氨酸组成的聚乙二醇GRGDS。原代肝细胞通过聚乙二醇GRGDS处理分散，取决于聚乙二醇的大小。经PEG-GRGDS处理的肝细胞存活率提高，细胞骨架组织形成，白蛋白产生和药物代谢活性得到很好的表达。因此，成功地开发了一种新的细胞功能评估方法，即利用聚乙二醇单细胞培养技术创建非粘附性单细胞培养技术。

PEG-GRGDS consisting of polyethylene glycol and arginine-glycine-aspartic acid was prepared. Primary hepatocytes were dispersed by PEG-GRGDS treatment depending on a PEG size. The hepatocytes treated with PEG-GRGDS improved their viability with the cytoskeleton organization, and well expressed albumin production and drug metabolism activities. Therefore, a novel estimation method of cell functions by creating non-adhesive single cell culture technology using PEG-GRGDS was successfully developed.