Analysis of molecular mechanisms of neuropathic pain and neciceptive signaling

神经性疼痛和伤害性信号传导的分子机制分析

• 批准号: 21658096
• 负责人: OHTA Toshio
• 金额: $ 2.16万
• 依托单位: Tottori University (2010-2011)Hokkaido University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21658096/
• 关键词: 薬理; 疼痛; Caイメージング; TRPA1; 知覚神経細胞; 疼痛反応; 炎症性疼痛; ヒスタミン; TRPチャネル; 知覚神経

To elucidate functional significance of Transient Receptor potential V1(TRPV1) and TRPA1 which are expressed in sensory neurons as neuropathic and inflammatory pain signaling, fluorescent protein-fused expressing vectors were constructed and heterologously expressed in mammalian cells. Their functions were compared to those obtained from sensory neurons. Histamine and hydrogen sulfide, which are synthesized and released under inflammatory conditions, potenciated and stimulated these channels, and elicited pain signaling through the activation of TRPV1 and TRPA1, respectively. These results suggest that TRP channels are important molecular targets for pain-regulation.

为了阐明瞬时受体电位V1（TRPV1）和TRPA1在感觉神经元中作为神经病理性和炎性疼痛信号传导的功能意义，构建了荧光蛋白融合表达载体，并在哺乳动物细胞中异源表达。他们的功能进行了比较，从感觉神经元。组胺和硫化氢在炎症条件下合成和释放，增强和刺激这些通道，并分别通过激活TRPV1和TRPA1引发疼痛信号。这些结果表明TRP通道是痛觉调节的重要分子靶点。

项目成果

Molecular Cloning and Functional Characterization of Xenopus tropicalis Frog Transient Receptor Potential Vanilloid 1 Reveal Its Functional Evolution for Heat, Acid, and Capsaicin Sensitivities in Terrestrial Vertebrates

• DOI: 10.1074/jbc.m111.305698
• 发表时间: 2012-01-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ohkita, Masashi;Saito, Shigeru;Ohta, Toshio
• 通讯作者: Ohta, Toshio

Decreased calcium channel currents and facilitated epinephrine release in the calcium channel beta3 subunit-null mice.

钙通道β3亚基缺失小鼠中钙通道电流减少并促进肾上腺素释放。

• 发表时间: 2010
• 作者: 村上学;大場貴喜;根本隆行;柳田俊彦;太田利男;M.MURAKAMI
• 通讯作者: M.MURAKAMI

Xenopus Tropicalis TRPV1遺伝子クローニングと発現・機能解析

热带爪蟾TRPV1基因克隆、表达及功能分析

• 发表时间: 2010
• 作者: 大北真嗣;齋藤茂;今川敏明;高橋賢次;太田利男
• 通讯作者: 太田利男

H2S FUNCTIONS AS A NOCICEPTIVE MESSENGER THROUGH TRANSIENT RECEPTOR POTENTIAL ANKYRIN 1 (TRPA1) ACTIVATION

• DOI: 10.1016/j.neuroscience.2012.05.044
• 发表时间: 2012-08-30
• 期刊: NEUROSCIENCE
• 影响因子: 3.3
• 作者: Ogawa, H.;Takahashi, K.;Ohta, T.
• 通讯作者: Ohta, T.

両生類TRPV1遺伝子クローニングと機能解析によるポリモーダル感受性の進化

通过两栖动物 TRPV1 基因克隆和功能分析进行多模式易感性的演变

• 发表时间: 2012
• 作者: 大北真嗣;齋藤茂;高橋賢次;富永真琴;太田利男
• 通讯作者: 太田利男