In vivo imaging analysis for cellular and molecular mechanisms of body axis tuning

在果蝇发育过程中，雄性外生殖器在形成过程中会进行360 °顺时针旋转，但如果旋转不完全停止，则外生殖器的L/R轴会发生变形。影像学分析显示，生殖器的完全旋转需要生殖器周围上皮细胞的两步运动。我们发现，由细胞凋亡引发的二级旋转加速了初级旋转，以调整发育时间窗的形态建成速度。

In Drosophila development, male genitalia performs 360o clockwise rotation as it forms, but L/R axis of genitalia is deformed if the rotation is incompletely stopped. Imaging analysis reveals that full rotation of genitalia is required the two steps movement of collective cells in epithelial sheet surrounding genitalia. We found that the secondary rotation that was initiated by apoptosis accelerated the primary rotation to adjust the speed of morphogenesis for the developmental time window.