Regulatory mechanisms for the membrane traffic during mitosis

在真核细胞中，由脂质双分子层组成的多种囊泡细胞器通过囊泡转运交换其内容物。在囊泡运输中，膜的出芽和融合受到严格的调控。当细胞在有丝分裂过程中分裂时，囊泡细胞器应均匀地分离成两个子细胞。为此，细胞主动抑制膜融合，使囊泡细胞器变小并增加其数量。在这项工作中，我们已经证明了Plk1，一种已知在有丝分裂过程中起作用的丝氨酸/苏氨酸激酶，是有丝分裂过程中抑制膜融合所必需的，并确定了Plk1的候选靶点分子。

In the eukaryotic cells, many kinds of vesicular organelles which are composed of the lipid bilayer exchange their contents by a vesicle transport. In a vesicle transport, the budding and fusion of the membranes are tightly regulated. When a cell divides during mitosis, vesicular organelles should be separated equally into two daughter cells. To this end, cells actively inhibit the membrane fusion to make the vesicular organelles small and increase their number. In this work, we have shown that Plk1, a Ser/Thr kinase that is known to function during mitosis, is necessary for the inhibition of the membrane fusion during mitosis, and identified a candidate molecule for the target of Plk1.