The identification of invasion associated genes in glioma stem cell

胶质瘤干细胞样细胞被证明是侵袭性的，但其机制仍有待阐明。在这项研究中，我们证明了整合素在胶质瘤干细胞样细胞侵袭中的作用。测定整合素家族在CD 133+和-细胞中的表达谱。整合素α 3在CD 133+细胞中的表达高于CD 133-细胞。免疫组织化学显示整合素α 3在侵袭性胶质母细胞瘤细胞中的定位。整合素α 3的表达水平与胶质瘤细胞的侵袭活性有关。胶质瘤细胞系的迁移活性与ERK（细胞外信号调节激酶）1/2的磷酸化水平有关。我们的研究结果表明，内源性整合素α 3有助于胶质瘤干细胞样细胞的侵袭性，这使得整合素α 3作为胶质母细胞瘤抗侵袭治疗的主要候选者。

Glioma stem-like cells are shown to be invasive but its mechanism remains to be elucidated. In this study we demonstrate the function of integrin in glioma stem-like cell invasion. The expression profile of integrin family in CD133+ and - cells was determined. Integrin α3 was overexpressed in CD133+ cells compared with CD133- cells. Immunohistochemistry demonstrated the localization of integrin alpha3 in invading glioblastoma cells. The activity of glioma cell invasion was associated with the expression level of integrin α3. The migration activity of glioma cell lines was linked to the phosphorylation level of ERK (extracellular signal-regulated kinase) 1/2. Our results suggest that endogenous integrin alpha3 contributes the invasive nature of glioma stem-like cells, which renders integrin alpha3 as a prime candidate for anti-invasion therapy for glioblastoma.