The identification of invasion associated genes in glioma stem cell

胶质瘤干细胞侵袭相关基因的鉴定

• 批准号: 21689038
• 负责人: NAKADA Mitsutoshi
• 金额: $ 12.23万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21689038/
• 关键词: グリオーマ; 幹細胞; 浸潤; インテグリン

Glioma stem-like cells are shown to be invasive but its mechanism remains to be elucidated. In this study we demonstrate the function of integrin in glioma stem-like cell invasion. The expression profile of integrin family in CD133+ and - cells was determined. Integrin α3 was overexpressed in CD133+ cells compared with CD133- cells. Immunohistochemistry demonstrated the localization of integrin alpha3 in invading glioblastoma cells. The activity of glioma cell invasion was associated with the expression level of integrin α3. The migration activity of glioma cell lines was linked to the phosphorylation level of ERK (extracellular signal-regulated kinase) 1/2. Our results suggest that endogenous integrin alpha3 contributes the invasive nature of glioma stem-like cells, which renders integrin alpha3 as a prime candidate for anti-invasion therapy for glioblastoma.

胶质瘤干细胞具有侵袭性，但其机制尚不清楚。在这项研究中，我们论证了整合素在胶质瘤干细胞侵袭中的作用。检测整合素家族在CD133+和CD133-细胞中的表达。与CD133-细胞相比，整合素α3在CD133+细胞中高表达。免疫组织化学显示整合素α3在侵袭性胶质母细胞瘤细胞中的定位。胶质瘤细胞系的迁移活性与细胞外信号调节蛋白α1/2的磷酸化水平有关。我们的结果提示，内源性整合素α3参与了胶质瘤干细胞的侵袭性，从而使整合素α3成为抗胶质母细胞瘤抗侵袭治疗的首选药物。

项目成果

Secondary anaplastic astrocytomadeveloping in a young adult withautoimmune lymphoproliferativesyndrome(ALPS)

患有自身免疫性淋巴增殖综合征 (ALPS) 的年轻成人继发性间变性星形细胞瘤

• DOI: 10.1111/j.1365-2990.2010.01123.x
• 发表时间: 2011
• 期刊: Neuropathol ApplNeurobiol
• 作者: Kita;D.;Hayashi;Y.;Watanabe;T.;Korshunov;A.;von Deimling;A.;Nakada;M.;Kasahara;Y.;Zen;Y. and Hamada;J
• 通讯作者: J

The expression level of sphingosine-1-phosphate receptor type 1 is related to MIB-1 labeling index and predicts survival of glioblastoma patients

• DOI: 10.1007/s11060-009-0064-5
• 发表时间: 2010-05
• 期刊: Journal of Neuro-Oncology
• 影响因子: 3.9
• 作者: Y. Yoshida;M. Nakada;Tomoya Harada;Shingo Tanaka;T. Furuta;Y. Hayashi;D. Kita;N. Uchiyama;Y. Hayashi;J. Hamada

The mechanism of glioma invasion and anti-invasion therapy.

胶质瘤侵袭机制及抗侵袭治疗。

• 发表时间: 2010
• 作者: Miyamoto T;et al;Nakada M.
• 通讯作者: Nakada M.

Radiation induced cerebellar high grade glioma accompanied with meningioma and cavernoma 29 years after the treatment of medulloblastoma

髓母细胞瘤治疗29年后放射诱发的小脑高级胶质瘤伴有脑膜瘤和海绵体瘤

• 发表时间: 2010
• 期刊: J Neurooncol (in press)
• 作者: 吉龍澄子;吉田謙;岡村玲子;松本義久.;Kamide T
• 通讯作者: Kamide T

RNA Technologies and Their Applications

RNA技术及其应用

• 发表时间: 2010
• 作者: 三宅俊輔;山田洋司;武中正;梶原敏郎;上田麻里;村田伸一;岩井康典;本多数弥;吉田謙;田中英一;山崎秀哉;Nakada M
• 通讯作者: Nakada M