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The analysis of relations of the chemokines which human gliomas produce and the tumor infiltrating macrophage/Microglia

人胶质瘤产生的趋化因子与肿瘤浸润巨噬细胞/小胶质细胞的关系分析

基本信息

批准号：
21700394
负责人：
OKADA Makoto
金额：
$ 1.75万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700394/
关键词：
MCP-1 MCP-3 chemokine glioma macrophage monocyte chemotactic protein-1 monocyte chemotactic ptotein-3

项目摘要

The monocyte chemotactic protein 3 (MCP-3) belongs to the MCP subgroup of the CC chemokines and promotes chemotaxis of immune cells. MCP-1 is believed to play an important role in monocyte infiltration into tumor tissues ; however, the relationship between tumor-infiltrating macrophage/microglia (TIM/M) and the expression of chemo- kines has not been investigated in detail in human glioma samples ; therefore, we first examined the expression of several chemokines and chemokine receptors in human tumor cell lines, which included glioma lines, using real-time PCR. We found that several glioma lines expressed MCP-3 predominantly, and not MCP-1. In order to assess the significance of MCP-3 expression in human glioma tissues, we then examined the number of CD68+ TIM/M, the percentage of TIM/M in the total cell population, and the expression of MCP-1 and MCP-3 in glioma tissues. There was a correlation between the percentage of TIM/M and MCP-3 expression levels ; however, there was no correlation between the percentage of TIM/M and MCP-1 expression. There was no correlation between the number of TIM/M and prognosis of patients. These data indicate that tumor cell-derived MCP-3, but not MCP-1, facilitates the infiltration of macrophage/ microglia into tumor tissues. This is the first study that clearly compared the significance of MCP-3 with that of MCP-1 in the tumor infiltration rates of TIM/M.

单核细胞趋化蛋白3(MCP-3)属于CC趋化因子的MCP亚类，促进免疫细胞的趋化。单核细胞趋化蛋白-1(MCP-1)被认为在单核细胞向肿瘤组织的侵袭中起重要作用；然而，肿瘤浸润性巨噬细胞/小胶质细胞(TIM/M)与趋化因子在人脑胶质瘤中表达的关系尚未得到详细研究，因此，我们首先利用实时荧光定量聚合酶链式反应(Real-Time-PCR)检测了几种趋化因子和趋化因子受体在包括胶质瘤在内的人肿瘤细胞系中的表达。我们发现，几种胶质瘤细胞株主要表达MCP-3，而不表达MCP-1。为了评估MCP-3在人脑胶质瘤组织中的表达意义，我们检测了CD68+TIM/M的数量、TIM/M占细胞总数的百分比以及MCP-1和MCP-3在胶质瘤组织中的表达。TIM/M百分率与MCP-3表达水平呈正相关，而TIM/M百分率与MCP-1表达无相关性。TIM/M数与患者预后无相关性。这些数据表明，肿瘤细胞来源的MCP-3，而不是MCP-1，促进了巨噬细胞/小胶质细胞向肿瘤组织的渗透。这是首次明确比较MCP-3和MCP-1在TIM/M的肿瘤浸润率中的意义的研究。