Effective preservation of stem cells with novel developed cryoprotectant

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We synthesized carboxylated poly-L-lysine by the reaction of poly-L-lysine (PLL) with succinic anhydride for the cryoprotective agents of living cells. Culture medium solutions of PLL with 65 mol% of amino groups carboxylated showed excellent post-thaw survival efficiency of various cell lines and rat, mouse, human mesenchymal stem cells and mouse and human induced pluripotent stem cells. Thus those polyampholytes can replace DMSO as new materials for cryoprotective agents. We elucidated the mechanisms of cryoprotective properties of those polyampholytes. The mechanisms would be involved with the membrane protective activity and inhibition of rapid increase of osmolarity during freezing by ion trapping to those polyampholytes.