The effect of L-ascorbic acid on transcriptional regulation

我们研究了l -抗坏血酸（AsA）给药（0、5和100 mg/d）对ODS大鼠肝脏钠依赖性维生素C转运体（svct）和其他基因表达水平的影响。同样，我们检测了在培养基中添加AsA对大鼠原代肝细胞和HepG2转运蛋白表达的影响。此外，为了研究svct启动子区域与转录因子之间的相互作用，我们进行了ChiP IP和qPCR检测。结果，未给予AsA的大鼠肝脏中SVCT1水平升高，而给予100 mg/d AsA的大鼠肝脏中SVCT1水平降低，表明这些转运蛋白水平可能受到AsA给药量的影响。此外，这些转运蛋白水平与体内氧化还原有关。ChiP IP和qPCR检测显示C/EBP与SVCT2启动子区结合。

We investigated the effects of L-ascorbic acid(AsA) administration(0, 5 and 100 mg/day) on the expression levels of sodium-dependent vitamin C transporters(SVCTs) and that of other genes in ODS rat livers. Similarly, the effect of AsA addition into the medium on the transporters expressions in rat primary hepatocyte and HepG2 was examined. Moreover, to examine the interaction between the promoter regions of SVCTs and the transcriptional factors, ChiP IP & qPCR assay was conducted.As a result, the SVCT1 levels increased in the livers of rats not administered AsA and decreased in the livers of rats administered 100 mg/day AsA, indicating that these transporter levels can be influenced by the amount of AsA administered. Also, these transporter levels can be associated with redox in vivo. The ChiP IP & qPCR assay exhibited the binding between of C/EBP and the promoter region of SVCT2.