Studies on search and action mechanism of new functional substances of a constituent of Porphyra yezoensis

为了阐明Nori对大鼠脂肪吸收的影响，我们给大鼠灌胃Nori样品。样品通过加热菜籽油和Nori细粉(RNAO)获得。与对照组相比，血液中脂质浓度、RNAO均升高。因此，为了揭示该制剂的作用机理，我们用从Nori中提取的水溶性膳食纤维(卟啉)进行了脂质负荷试验，结果发现其脂质吸收受到了抑制。此外，还考察了这种影响是否依赖于Nori溶液的粘度。结果表明，它并不是为了通过粘度来促进脂肪的吸收。这些结果是通过测量血液中的血脂水平得出的。为了更准确地评估，下一项实验检查了淋巴的吸收情况。结果表明，淋巴流量和磷脂浓度未受影响，但脂质浓度升高。这些观察结果提示，RNAO可能促进脂质的吸收。此外，我们还对Nori中的胰岛素样作用物质进行了分离和纯化，但没有得到活性物质的鉴定。

In order to clarify the effect on the lipid absorption of Nori, we administered Nori sample to rats. The sample was obtained by heating rapeseed oil and Nori fine powder (RNAO). The lipid concentration in the blood compared to the control group, RNAO were high. Therefore, in order to reveal the agent, we subjected to a lipid load test using water-soluble dietary fiber (porphyran) extracted from Nori, unexpectedly lipid absorption was inhibited. Moreover, this effect was investigated whether it was depended on the viscosity of Nori solution. It was shown that it is not intended to promote lipid absorption by the viscosity. These results were measured lipid level in blood. In order to evaluate more accurately, the next experiment was examined absorption into the lymph. As a result, lymph flow and the phospholipid concentration were not influenced, but lipid concentration became higher. These observations suggest that RNAO may promote the lipid absorption. Further, we advanced the separation and purification of the insulin-like action substance from Nori, but did not lead to identification of the active substance