MARcode : a prediction program for nuclear matrix attachment regions

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We focused on SP120, one of the nuclear matrix-associated proteins to develop MARcode, a prediction program for matrix attachment regions (MAR). It is suggested that SP120 is involved in the neuronal differentiation through its binding to DNA topoisomerase (topo) IIβ. We showed that SP120 specifically bound to AT-rich topoIIβ action sites. We performed a domain analysis for MAR-binding activity of SP120 and found that RG-domain had the activity. We showed the evidence that SP120 directly bound to AT patches (short stretches of consecutive A and T bases). We performed a genome-wide analysis to identify in vivo SP120 binding sites. We will further analyze the identified regions to develop the MARcode program.