Analysis of Transcriptional Regulation of Genome by Pathogenicity Transcription Factor LeuO.

为了深入了解LeuO的调控作用(S)，我们试图使用基因组SELEX系统确定一整套调控靶点。在大肠杆菌基因组上共鉴定出140个LeuO结合位点，其中125个是新鉴定的。结合我们以前发现的LeuO参与多药外排的发现，揭示了LeuO调节许多生物被膜形成的基因，包括菌毛形成的基因。

To get insights into the regulatory role(s) of LeuO, attempts were made to identify the whole set of regulation targets using Genomic SELEX system. A total of 140 LeuO-binding sites were identified on the E.coli genome, 125 of which were newly identified. Taken together with our previous finding of the involvement of LeuO in mutli-drug efflux, it was revealed that LeuO regulates a number of genes for biofilm formation including the genes for fimbriae formation.