Functional analyses of antisense transcripts in the epigenetic regulation of gene expression

基因体内的DNA甲基化被认为会阻碍转录延伸，但基因DNA甲基化的因果作用仍然基本上未知。在这项研究中，我在35碱基对分辨率下生成了拟南芥野生型和DNA甲基转移酶缺失突变体的DNA甲基化谱（甲基组）以及正义和反义转录谱（转录组）。甲基组和转录组的组合分析表明，一些正/反义转录物与体甲基化有关。我还研究了身体甲基化和小rna之间的关系。

DNA methylation within the gene body is thought to impede transcriptional elongation, but the causal role of genic DNA methylation remains essentially unknown. In this research, I have generated DNA methylation profile (methylome) as well as sense and antisense transcription profiles (transcriptome), at 35-base pair resolution, of the wild-type and the loss-of DNA methyltransferase mutant of Arabidopsis. Combinational analyses of the methylomes and the transcriptomes have indicated that some sense/antisense transcripts are associated with body methylation. I also examined correlation between body methylation and small RNAs.