Functional analyses of antisense transcripts in the epigenetic regulation of gene expression

基因体内的DNA甲基化被认为阻碍了转录延伸，但基因DNA甲基化的因果作用基本上仍然未知。在这项研究中，我已经产生了DNA甲基化谱（甲基化组）以及正义和反义转录谱（转录组），在35个碱基对的分辨率，野生型和拟南芥的DNA甲基转移酶突变体的损失。甲基化组和转录组的组合分析表明，一些有义/反义转录本与身体甲基化有关。我还研究了身体甲基化和小RNA之间的相关性。

DNA methylation within the gene body is thought to impede transcriptional elongation, but the causal role of genic DNA methylation remains essentially unknown. In this research, I have generated DNA methylation profile (methylome) as well as sense and antisense transcription profiles (transcriptome), at 35-base pair resolution, of the wild-type and the loss-of DNA methyltransferase mutant of Arabidopsis. Combinational analyses of the methylomes and the transcriptomes have indicated that some sense/antisense transcripts are associated with body methylation. I also examined correlation between body methylation and small RNAs.