Analysis of chromatin remodeling machinery required for the regulation of DNA replication

为了分析INO 80染色质重塑复合物在DNA复制和维持基因组稳定性中的功能，我们建立了降解决定子酵母菌株，其中Ino 80和Arp 8被诱导消化。我们发现INO80复合物的损伤导致基因组不稳定。我们还显示了INO 80复合物与DNA复制因子、染色质蛋白和核孔复合物的生理和遗传相互作用。这些结果对于揭示INO 80复合物在DNA复制和维持基因组稳定性中的分子机制具有重要意义。

To analyze the function of the INO80 chromatin remodeling complex in DNA replication and in the maintenance of genome stability, we established Degron yeast strains in which Ino80 and Arp8 are inducibly digested. We showed that the impairment of the INO80 complex caused genome instability. We also showed physiological and genetic interactions of the INO80 complex with DNA replication factors, chromatin proteins, and nuclear pore complex. Theseresults are important to reveal molecular mechanisms of the INO80 complex in DNAreplication and the maintenance of genome stability.