Modulation of rank-ordered recruitment of motor units in the jaw-closing movement by leak K+ channels

通过泄漏 K 通道调节闭颌运动中运动单位的有序募集

• 批准号: 22300127
• 负责人: KANG Youngnam
• 金额: $ 11.81万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22300127/
• 关键词: 三叉神経運動ニューロン; 漏洩Kチャネル; NO; 序列動員; α運動ニューロン; γ運動ニューロン; 漏洩カリウムチャネル; サイズの原理; 等尺性収縮; 三叉神経; 閉口筋; 運動ニューロン; TASKチャネル; 一酸化窒素; 入力抵抗; 三叉神経運動核; 等尺性筋収縮

The immunohistochemical analysis of motoneurons (MNs) in the trigeminal motor nucleus that innervates jaw-closing muscles revealed that TASK1 and TASK3 are complementary expressed in the soma and dendrites of MNs, respectively. The real-time RT PCR revealed that large MNs (>35 microm) expressed more TASK1/cell and TASK3 compared to small MNs (<20 microm). Consequently, larger MNs had smaller input resistance (IR) and more hyperpolarized resting membrane potentials (RMP) compared to smaller MNs. PKG activation enhanced TASK1 activity while it inhibited TASK3 activity. As the MN size becomes smaller, PKG activation more decreased the IR and hyperpolarized the RMP. The frequency distribution of sizes of alpha-MNs was found to display a bimodal distribution composed of smaller and larger alpha-MNs while gamma-MNs displayed a unimodal patter of smaller size. These finding provide crucial molecular and cellular bases of the rank-ordered recruitment of motor units in the jaw-closing movement.

对支配下颌闭合肌的三叉神经运动核中运动神经元（MNs）的免疫组织化学分析显示，TASK 1和TASK 3分别在MNs的索马体和树突中互补表达。实时RT PCR显示，与小MN（<20 μ m）相比，大MN（>35 μ m）表达更多的TASK 1/细胞和TASK 3。因此，与较小的MNs相比，较大的MNs具有较小的输入电阻（IR）和更多的超极化静息膜电位（RMP）。PKG激活增强TASK 1活性，同时抑制TASK 3活性。随着MN尺寸变小，PKG激活更多地降低IR并使RMP超极化。发现α-MN的大小的频率分布显示由较小和较大的α-MN组成的双峰分布，而γ-MN显示较小尺寸的单峰图案。这些发现提供了关键的分子和细胞基础的排名有序募集的运动单位在下颌闭合运动。

项目成果

Intercolumnar and intracolumnar desynchronization by GABAB receptor-mediated presynaptic inhibition in the rat barrel cortex.

GABAB 受体介导的大鼠桶状皮层突触前抑制导致柱间和柱内去同步。

• 发表时间: 2011
• 作者: 齋藤充;佐藤元;豊田博紀;田中琢真;小林真之;Daniel Althof;Akos Kulik;青柳富誌生;重本隆一;姜英男
• 通讯作者: 姜英男

三叉神経中脳路核ニューロンにおいてグルタミン酸作動性入力を無効化するイオン機構

中和三叉神经中脑束核神经元谷氨酸能输入的离子机制

• 发表时间: 2010
• 作者: 川嵜康大;齋藤充;佐藤元;豊田博紀;姜英男
• 通讯作者: 姜英男

Recruitment of masseter motoneurons by the presumed spindle Ia inputs. In: Breathe, Walk and Chew - The Neural Challenge: Part I (Progress in Brain Research, Vol. 187)(Gossard JP, Dubuc R, Kolta A, Eds.)(163-171)

通过假定的纺锤体 Ia 输入募集咬肌运动神经元。

• 发表时间: 2010
• 作者: Kang Y;Saito M;Toyoda H;Sato H
• 通讯作者: Sato H

Protein kinase C dependent modulation of spike firing in mesencephalic trigeminal nucleus neurons

中脑三叉神经元尖峰放电的蛋白激酶 C 依赖性调节

• 发表时间: 2010
• 作者: Chung;G;Kang;Y.;et al.
• 通讯作者: et al.

Enhanced desensitization followed by unusual resensitization in GABAA receptors in phospholipase C-related catalytically inactive protein-1/2 double-knockout mice

• DOI: 10.1007/s00424-014-1511-5
• 发表时间: 2014-04
• 期刊: Pflügers Archiv - European Journal of Physiology
• 作者: H. Toyoda;M. Saito;Hajime Sato;Takuma Tanaka;Takeo Ogawa;H. Yatani;Tsutomu Kawano;T. Kanematsu;M. Hirata;Youngnam Kang