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Effects of intermediate frequency magnetic fields on central nervous system and immune systems in mice.

中频磁场对小鼠中枢神经系统和免疫系统的影响。

基本信息

批准号：
22300255
负责人：
KUNUGITA Naoki
金额：
$ 12.23万
依托单位：
National Institute of Public Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Recently, a cooking appliance based on the principle of electromagnetic induction has come to be used domestically on a widespread basis; this induction heating cooking hob mainly generates intermediate-frequency magnetic fields (IF-MF). However, whether electromagnetic fields originating from household appliances represent a health risk remains uncertain. We investigated the effect of IF-MF on the expressions of memory function-related genes and related transduction molecules in the mouse hippocampus. Male and female C57BL/6J mice were allotted to a control (sham-exposed), an exposure, or a recovery (one week after exposure) group and were exposed to IF-MF (21 kHz, 3.8 mT) one hour per day for 2 weeks. Twenty-four hour after final exposure, the expression levels of memory function-related genes and the mRNA levels for signal transduction pathway molecules in the hippocampi were examined using real-time RT-PCR. The relative mRNA expression levels of the N-methyl-D aspartate (NMDA) receptor subunits NR1, NR2A, and NR2B as well as transcription factors (calcium/calmodulin-dependent protein kinase [CaMK] -IV, cyclic AMP responsive element binding protein [CREB] -1) and neurotrophins (nerve growth factor [NGF], and brain-derived neurotrophic factors [BDNF]) were not significantly altered in the IF-MF-exposed mice. We also examined the morphology of the hippocampus using a histological analysis, but no changes in the IF-MF-exposed mice were seen. The present findings suggest that IF-MF exposure may not affect cognitive function in the present animal model.

近来，基于电磁感应原理的烹饪器具已经在国内广泛使用;这种感应加热烹饪灶具主要产生中频磁场（IF-MF）。然而，家用电器产生的电磁场是否会对健康构成风险仍不确定。本研究探讨了干扰素-微乳对小鼠海马记忆功能相关基因及相关转导分子表达的影响。将雄性和雌性C57 BL/6 J小鼠分配到对照组（假暴露）、暴露组或恢复组（暴露后1周），并每天暴露于IF-MF（21 kHz，3.8 mT）1小时，持续2周。在最终暴露后24小时，使用实时RT-PCR检测海马中记忆功能相关基因的表达水平和信号转导通路分子的mRNA水平。在IF-MF暴露小鼠中，N-甲基-D天冬氨酸（NMDA）受体亚单位NR 1、NR 2A和NR 2B以及转录因子（钙/钙调蛋白依赖性蛋白激酶[CaMK] -IV、环AMP反应元件结合蛋白[CREB]-1）和神经营养因子（神经生长因子[NGF]和脑源性神经营养因子[BDNF]）的相对mRNA表达水平未发生显著变化。我们还使用组织学分析检查了海马的形态学，但在IF-MF暴露的小鼠中没有观察到变化。目前的研究结果表明，IF-MF暴露可能不会影响本动物模型的认知功能。