Cryo-bioimaging by using coherent X-ray diffraction

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We revised the established theory of the structure of human chromosomes by X-ray scattering experiments conducted using SPring-8. Experimental data indicate irregularly folded nucleosome fibers without a 30-nm chromatin structure or regularly folding of chromatin fibers. We also proposed a new coherent imaging method using a confined illumination, which can be applicable also to frozen-hydrated biological samples with extended ice layer, to overcome the limitation of conventional data analysis algorithm in X-ray diffraction microscopy.