Protein Structure Analysis by Second-order Nonlinear Spectroscopy

我表明，一个新开发的二阶非线性光谱技术，使我能够绘制这样的蛋白质的拓扑图。独特的是，人们可以使用这种二阶非线性光谱来确定表面上分子的“向上”与“向下”排列。在我的新颖但简单的方法中，在将蛋白质固定在玻璃表面之前，将“对齐指示剂”引入蛋白质的每个片段，并且每个片段的上下对齐是非线性光谱确定的。这种方法非常强大，对于X射线和NMR都不适用的蛋白质非常有用。

I show that a newly-developed second-order nonlinear spectroscopic technique allows me to draw topology diagrams of such proteins. Uniquely, one can determine "up" versus "down" alignment of molecules on a surface using this second-order nonlinear spectroscopy. In my novel but simple approach, an "alignment indicator" is introduced to each segment of a protein before immobilizing the protein on a glass surface, and the up versus down alignment of each segment is nonlinear-spectroscopically determined. This approach is very powerful and useful for proteins to which neither X-ray nor NMR is applicable.