Protein Structure Analysis by Second-order Nonlinear Spectroscopy

我展示了一种新开发的二阶非线性光谱技术，使我能够绘制这种蛋白质的拓扑图。独一无二的是，人们可以使用这种二阶非线性光谱学来确定分子在表面上的“向上”或“向下”排列。在我新颖而简单的方法中，在将蛋白质固定在玻璃表面之前，在蛋白质的每一段上引入一个“排列指示器”，每一段的上下排列是由非线性光谱确定的。这种方法对于X射线和核磁共振都不适用的蛋白质来说是非常强大和有用的。

I show that a newly-developed second-order nonlinear spectroscopic technique allows me to draw topology diagrams of such proteins. Uniquely, one can determine "up" versus "down" alignment of molecules on a surface using this second-order nonlinear spectroscopy. In my novel but simple approach, an "alignment indicator" is introduced to each segment of a protein before immobilizing the protein on a glass surface, and the up versus down alignment of each segment is nonlinear-spectroscopically determined. This approach is very powerful and useful for proteins to which neither X-ray nor NMR is applicable.