Studies on molecular mechanisms for radish male sterility/fertilityrestoration system and its diversification

为了揭示萝卜雄性不育/育性恢复的分子机制，以及与此相关的线粒体和核基因的进化，进行了一系列实验。分子研究表明，萝卜育性恢复基因pprB的蛋白产物与线粒体雄性不育基因orf138的mRNA结合，并通过其结构的改变抑制其翻译。线粒体基因组学研究表明，orf138是由正常细胞质和Ogura细胞质之间线粒体基因组的结构变化意外产生的。

In order to reveal molecular mechanisms underlying malesterility/fertility restoration in radish and the evolution of mitochondrial and nuclear genesrelevant to the phenomena, a series of experiments were conducted. Molecular studyrevealed that protein product from the gene pprB (one of the fertility restorer genes inradish) binds to mRNA of the mitochondrial male-sterility gene orf138 and inhibit itstranslation through the structural change of mRNA. Mitochondrial genomics revealedthat orf138 was created accidentally by the structural changes of mitochondrial genomesbetween normal and Ogura cytoplasm.