Characterization and modification of molecular mechanism for transcriptional regulation of the degradation enzyme system of an environmental pollutant

环境污染物降解酶系统转录调控分子机制的表征和修饰

• 批准号: 22380050
• 负责人: FUKUDA Masao
• 金额: $ 11.48万
• 依托单位: Nagaoka University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380050/
• 关键词: 環境微生物; 転写制御機構; 二成分制御系; PCB; Rhodococcus; ビフェニル

Rhodococcus jostii RHA1 is a strong degrader of the environmental pollutant, polychlorinated biphenyls (PCBs). Induction of its degradation enzymes requires an inducing substrate and is conducted by a couple of two-component transcriptional regulators, BphS1T1 and BphS2T2. Mechanism of transcriptional regulation of BphS1T1 and BphS2T2 were examined to develop a strain whose PCB-degradation enzymes are induced by PCBs themselves. Phosphorylation of BphT (BphT1/T2) in the presence of the inducing substrate was indicated. A novel 24-bp consensus sequence was shared among the degradation enzyme gene operon promoters and had necessary and sufficient function for transcriptional induction by BphST, suggesting that the 24-bp sequence serves the binding site for the transcriptional complex including BphT.

Jostii红球菌RHA 1是一种对环境污染物多氯联苯（PCBs）具有很强降解能力的细菌。其降解酶的诱导需要诱导底物，并由一对双组分转录调节因子BphS 1 T1和BphS 2 T2进行。研究了BphS 1 T1和BphS 2 T2的转录调控机制，以开发其PCB降解酶由PCB本身诱导的菌株。在诱导底物的存在下，BphT（BphT 1/T2）的磷酸化被指示。降解酶基因操纵子启动子之间共享一个新的24-bp的共有序列，并且具有BphST转录诱导所必需的和足够的功能，这表明24-bp的序列为包括BphT的转录复合物的结合位点服务。

项目成果

PCB分解菌の育種

PCB降解菌的选育

• 发表时间: 2010
• 作者: Nishiyama;E.;Y. Ohtsubo;Y. Nagata;and M. Tsuda;福田雅夫
• 通讯作者: 福田雅夫

The 24-bp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter

负责调节混合启动子中 BphS1T1 双组分系统的 24 bp 共​​有序列

• DOI: 10.1016/j.jbiosc.2011.10.021
• 发表时间: 2012
• 期刊: J. Biosci. Bioeng
• 作者: J. Shimodaira;Y. Furusawa;Y. Miyazawa;D. Kasai;K. Miyauchi;E. Masai;M. Fukuda
• 通讯作者: M. Fukuda

The essential sequence for transcriptional regulation of biphenyl/polychlorinated biphenyls-degradation gene promoters in Rhodococcus jostii RHA1

红球菌 RHA1 联苯/多氯联苯降解基因启动子转录调控的必需序列

• 发表时间: 2011
• 作者: Shimodaira J;Miyazawa Y;Furusawa Y;Takeda H;Kasai D;Miyauchi K;Masai E;Fukuda M
• 通讯作者: Fukuda M

長岡技術科学大学生物系研究室環境微生物工学研究室

长冈工业大学生物实验室环境微生物工学实验室

Rhodococcus jostii RHA1 の BphST 二成分制御系による転写誘導機構の解明

红球菌 RHA1 的 BphST 双组分调控系统阐明转录诱导机制

• 作者: M. Oku;Y. Ichiki;A. Shiraishi;T. Takahara;T. Maeda,and Y. Sakai;下平潤
• 通讯作者: 下平潤