Characterization and modification of molecular mechanism for transcriptional regulation of the degradation enzyme system of an environmental pollutant
Characterization and modification of molecular mechanism for transcriptional regulation of the degradation enzyme system of an environmental pollutant
批准号:
22380050
负责人:
FUKUDA Masao
金额:
$11.48万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012
中文摘要
Jostii红球菌RHA 1是一种对环境污染物多氯联苯(PCBs)具有很强降解能力的细菌。其降解酶的诱导需要诱导底物,并由一对双组分转录调节因子BphS 1 T1和BphS 2 T2进行。研究了BphS 1 T1和BphS 2 T2的转录调控机制,以开发其PCB降解酶由PCB本身诱导的菌株。在诱导底物的存在下,BphT(BphT 1/T2)的磷酸化被指示。降解酶基因操纵子启动子之间共享一个新的24-bp的共有序列,并且具有BphST转录诱导所必需的和足够的功能,这表明24-bp的序列为包括BphT的转录复合物的结合位点服务。
英文摘要
Rhodococcus jostii RHA1 is a strong degrader of the environmental pollutant, polychlorinated biphenyls (PCBs). Induction of its degradation enzymes requires an inducing substrate and is conducted by a couple of two-component transcriptional regulators, BphS1T1 and BphS2T2. Mechanism of transcriptional regulation of BphS1T1 and BphS2T2 were examined to develop a strain whose PCB-degradation enzymes are induced by PCBs themselves. Phosphorylation of BphT (BphT1/T2) in the presence of the inducing substrate was indicated. A novel 24-bp consensus sequence was shared among the degradation enzyme gene operon promoters and had necessary and sufficient function for transcriptional induction by BphST, suggesting that the 24-bp sequence serves the binding site for the transcriptional complex including BphT.
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PCB分解菌の育種
PCB降解菌的选育
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Nishiyama, E., Y. Ohtsubo, Y. Nagata, and M. Tsuda, 福田雅夫]
通讯作者:
福田雅夫
The 24-bp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter
负责调节混合启动子中 BphS1T1 双组分系统的 24 bp 共有序列
DOI:
10.1016/j.jbiosc.2011.10.021
发表时间:
2012
期刊:
J. Biosci. Bioeng
影响因子:
--
作者:
[J. Shimodaira, Y. Furusawa, Y. Miyazawa, D. Kasai, K. Miyauchi, E. Masai, M. Fukuda]
通讯作者:
M. Fukuda
The essential sequence for transcriptional regulation of biphenyl/polychlorinated biphenyls-degradation gene promoters in Rhodococcus jostii RHA1
红球菌 RHA1 联苯/多氯联苯降解基因启动子转录调控的必需序列
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Shimodaira J, Miyazawa Y, Furusawa Y, Takeda H, Kasai D, Miyauchi K, Masai E, Fukuda M]
通讯作者:
Fukuda M
長岡技術科学大学生物系研究室環境微生物工学研究室
长冈工业大学生物实验室环境微生物工学实验室
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Rhodococcus jostii RHA1 の BphST 二成分制御系による転写誘導機構の解明
红球菌 RHA1 的 BphST 双组分调控系统阐明转录诱导机制
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[M. Oku, Y. Ichiki, A. Shiraishi, T. Takahara, T. Maeda,and Y. Sakai, 下平潤]
通讯作者:
下平潤
共 14 条
Studies on the transcriptional regulatory system for sensing of environmental pollutants
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批准号:12460043
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.3万
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财政年份:2000
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负责人:FUKUDA Masao
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依托单位:
海外基金