Development of micropropagation method by inducing embryogenic totipotent cells from non-pollen Cryptomeria

我们利用无花粉柳杉(Sugi)无性系的体外培养建立了微繁技术。在所测试的细胞分裂素中，玉米素对芽的诱导相对有效。不育无性系福岛5号在玉米素+BAP+玉米素+BAP+1/2LP+5g/L活性炭+1/2LP+BAP+BAP+1/2LP+5g/L活性碳+1/2LP+BAP+1/2LP+5g/LP5+BAP+BAP+1/2LP+5g/LP5+BAP+BAP+1/2LP+5g/100g/100g/100g/100g/100g/LP值的培养基上可诱导出芽。不定芽在含有2 3.8BAPM(5 mg/L)和0.044μM(0.0 1 mg/L)BAP2 3.8μM(5 mg/L)的RIM培养基上生根。这些材料将用于雄性不育甜柏的微繁殖，以减少空气中的花粉。

We developed the micropropagation technique using in vitro culture of non-pollen Cryptomeria (sugi) clones. Among the cytokinin tested, zeatin was relatively effective for bud induction. More buds were induced in the medium containing zeatin than that containing BAP with male sterile clone Fukushima 5. Shoots were developed from buds on the medium 1/2 LP containing 5 g/l activated charcoal. Shoots were rooted on the RIM medium containing 23.8 μM (5 mg/l) Cl-IAA and 0.044 μM (0.01 mg/l) BAP. These materials will be used for micropropagation of male sterile sugi cedar in order to reduce its pollen in the air.