Role of chemokines in immune response of teleost fish

研究了两种趋化因子IL-8和CXCL13在牙鲆体内的趋化活性。IL-8和CXCL13分别选择性诱导中性粒细胞和B淋巴细胞迁移。我们还生产了针对这些趋化因子的特异性抗体，并开发了免疫测定法来估计这些趋化因子的数量。比目鱼白细胞在不同病原菌的刺激下产生不同的IL-8。我们认为IL-8的产生与牙鲆感染性疾病免疫反应的多样性有关。

We revealed chemotactic activity of two types of chemokines, IL-8 and CXCL13 in Japanese flounder. IL-8 and CXCL13 induced selectively the migration of neutrophils and B lymphocytes, respectively. We also produced specific antibodies to these chemokines and developed immunoassays to estimate the quantity of these chemokines. Flounder leukocytes showed various responses in their IL-8 production that induced by stimulation with several species of bacterial pathogens. We suggested that IL-8 production is relevant to diversity of immune response observed in infectious diseases of Japanese flounder.