Fundamental research on the development of high sensitive detection of target molecules by the use of self-assembled phospholipid layer possessing fluidity

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We aimed to develop the novel high sensitive detection method of target molecules utilizing the formation of rotaxane structure on the surface of self-assembled phospholipid layer possessing fluidity. It is well-known that the rotaxane structure is formed by mixing cyclodextrin (CD) and polyethylene glycol (PEG) in water, in which many CD units are threaded by PEG. Two kinds of CD derivatives, which possessed Cy3 or Cy5 to induce the fluorescence resonance energy transfer (FRET), were immobilized on the surface of self-assembled phospholipid layer. It was observed that the fluorescence intensity changed by adding PEG aqueous solution to the film immobilizing CD derivatives possessing Cy3 or Cy5 due to the formation of rotaxane structure. It is hoped that the high sensitive detection of PEG might be developed by this method.