Fundamental research on the development of high sensitive detection of target molecules by the use of self-assembled phospholipid layer possessing fluidity

利用具有流动性的自组装磷脂层开发高灵敏度检测目标分子的基础研究

• 批准号: 22390009
• 负责人: KONDO Shin-ichi
• 金额: $ 11.23万
• 依托单位: Gifu Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390009/
• 关键词: リン脂質自己組織化膜; 流動性; プラズマ; ロタキサン構造

We aimed to develop the novel high sensitive detection method of target molecules utilizing the formation of rotaxane structure on the surface of self-assembled phospholipid layer possessing fluidity. It is well-known that the rotaxane structure is formed by mixing cyclodextrin (CD) and polyethylene glycol (PEG) in water, in which many CD units are threaded by PEG. Two kinds of CD derivatives, which possessed Cy3 or Cy5 to induce the fluorescence resonance energy transfer (FRET), were immobilized on the surface of self-assembled phospholipid layer. It was observed that the fluorescence intensity changed by adding PEG aqueous solution to the film immobilizing CD derivatives possessing Cy3 or Cy5 due to the formation of rotaxane structure. It is hoped that the high sensitive detection of PEG might be developed by this method.

我们旨在利用具有流动性的自组装磷脂层表面形成轮烷结构，开发一种新的高灵敏度的靶分子检测方法。众所周知，轮烷结构是由环糊精（CD）和聚乙二醇（PEG）在水中混合形成的，其中许多环糊精单元是由PEG串成的。将含有Cy3或Cy5诱导荧光共振能量转移（FRET）的两种CD衍生物固定在自组装磷脂层表面。观察到在含有Cy3或Cy5的CD膜固定化衍生物中加入PEG水溶液后，由于形成轮烷结构，荧光强度发生了变化。希望该方法能开发出高灵敏度的聚乙二醇检测方法。

项目成果

Cold plasma techniques for pharmaceutical and biomedical engineering, Biomedical Engineering-Trends in Materials Science (edited by Anthony N. Laskovski)

用于制药和生物医学工程的冷等离子体技术，生物医学工程-材料科学趋势（安东尼·N·拉斯科夫斯基编辑）

• 发表时间: 2011
• 作者: Yasushi Sasai;Shin-ichi Kondo;Yukinori Yamauchi;Masayuki Kuzuya
• 通讯作者: Masayuki Kuzuya

Immobilization of Proteins onto the Self-Assembled Phospholipid Layer Fabricated by Plasma-Assisted Method

等离子体辅助法制备的自组装磷脂层上固定蛋白质

• DOI: 10.2494/photopolymer.24.467
• 发表时间: 2011
• 期刊: Journal of Photopolymer Science and Technology
• 影响因子: 0.8
• 作者: Shin-ichi Kondo;Yasushi Sasai;Yukinori Yamauchi;Masayuki Kuzuya
• 通讯作者: Masayuki Kuzuya

Application of Self-Assembled Phospholipid Layer Fabricated by Plama-Assisted Method as a Biointerface

等离子体辅助法制备的自组装磷脂层作为生物界面的应用

• 发表时间: 2012
• 作者: S.Kondo;M.Kuzuya
• 通讯作者: M.Kuzuya

プラズマを利用して構築したリン脂質自己組織化膜の流動性の評価とその応用

等离子体构建磷脂自组装膜的流动性评价及其应用

• 发表时间: 2012
• 作者: Shin-ichi Kondo;Masayuki Kuzuya;近藤伸一
• 通讯作者: 近藤伸一

高分子表面に構築したリン脂質自己組織化膜への抗チトクロムC抗体固定化と活性評価

聚合物表面磷脂自组装膜抗细胞色素C抗体的固定化及活性评价

• 发表时间: 2011
• 作者: 蛭間貴子;笹井泰志;近藤伸一;山内行玄;葛谷昌之
• 通讯作者: 葛谷昌之