Molecutar anatomy and medicinal application of laminins, a basement membrane protein

层粘连蛋白是基底膜（一种薄的细胞外基质）的主要成分，具有多种生物活性。我们的目标是鉴定层粘连蛋白的活性序列，并将生物活性肽用于医疗应用，包括抗癌和伤口愈合药物，DDS和生物材料。我们已经鉴定了各种生物活性肽使用超过1000合成肽衍生的层粘连蛋白。我们还鉴定了活性肽的细胞受体，并评估了它们的细胞信号传导。我们还证明了肽偶联多糖（壳聚糖、海藻酸盐和琼脂糖）基质作为细胞培养和组织工程的生物材料是有用的。此外，使用多种受体结合肽的混合肽基质促进了协同效应。利用与组织相适应的层粘连蛋白衍生多肽的组合，混合多肽-多糖基质可作为组织工程的功能性生物材料。

Laminins are a major component of the basement membrane, a thin extracellular matrix, and have diverse biological activities. Our goal is to identify active sequences from laminins and to use the biologically active peptides for medical application including anti-cancer and wound healing drugs, DDS, and biomaterials. We have identified various biologically active peptides using more than 1000 synthetic peptides derived from the laminins. We also identified cellular receptors of the active peptides and evaluated their cellular signaling. We also demonstrated that peptide-conjugated polysaccharide (chitosan, alginate, and agarose) matrices are useful as a biomaterial for cell culture and tissue engineering. Further, mixed peptide-matrices using several types of receptor-binding peptides promoted synergistic effects. Using a combination of tissue-appropriate laminin-derived peptides, the mixed peptide-polysaccharide matrices may serve as a functional biomaterial for tissue engineering.