CXCL14/BRAK is a multifunctional tumor suppressor

目的：本研究的目的是确定趋化因子BRAK/ CXCL14转基因（Tg）小鼠是否对肿瘤发生、肿瘤生长和肿瘤转移具有抗性。方法：野生型C57BL/6 （Wt）或Tg小鼠腹腔注射12 mg/kg体重的偶氮氧甲烷，然后在饮用水中间歇性添加3%葡聚糖硫酸钠。我们还通过小鼠的皮下或尾静脉注射肿瘤细胞。结果：Wt组与Tg组小鼠体重无显著差异，但结直肠癌发生率比Tg组高近10倍（P<0.001）。Tg组移植瘤的大小和肺转移灶数量均明显低于Wt组。注射Tg小鼠的黑色素瘤细胞存活率明显高于Wt组。结论：Tg小鼠对肿瘤发生、肿瘤生长和肿瘤细胞转移具有抵抗作用，说明BRAK/ CXCL14具有多种肿瘤抑制作用。

Objectives: The aim of this study was to determine whether or not chemokine BRAK/ CXCL14 transgenic (Tg) mice would show resistance to carcinogenesis, tumor growth and tumor metastasis. Methods: Wild type C57BL/6 (Wt) or Tg mice were injected intraperitoneally with 12 mg/kg body weight azoxymethane followed by intermittent addition of 3 % dextran sodium sulfate in the drinking water. We also injected tumor cells subcutaneously or via a tail vein of the mice. Results: No difference of body weight was observed between Wt and Tg mice but the rate of colorectal cancer was nearly ten times higher (P<0.001) in Wt mice than in Tg ones. Size of transplanted tumors and the number of metastatic foci on the lung of the animals were always significantly lower in the Tg that those in the Wt. Survival rates of the melanoma cell injected Tg mice were significantly higher than those of Wt. Conclusion: The Tg mice showed resistance to carcinogenesis, tumor growth and tumor cell metastasis, indicating BRAK/ CXCL14 has multifunctional effects on tumor suppression.