Development of a new treatment for trigeminal neuron sensitization

将荧光素标记的肉毒杆菌毒素重链注射到大鼠面部皮肤，在同侧三叉神经节内发现肉毒杆菌毒素重链。在对侧或唯一的荧光素注射剂中均未发现标记肉毒毒素重链。这种摄取被秋水仙碱抑制，从而阻断了轴突的运输。皮内注射肉毒杆菌毒素可减轻眶下神经收缩引起的热痛过敏。背根神经节直接注射肉毒杆菌毒素可逆转坐骨神经卡压所致的戒断阈值降低。

Fluorescein labeled botulinum toxin heavy chain which works for endocytosis was injected into rat face skin and found in ipsilateral trigeminal ganglion. The labeled botulinum toxin heavy chain was not found on the contralateral side or in the only fluorescein injection. The uptake was inhibited by colchicine treatment, which blocks axonal transport. Intradermal injection of botulinum toxin alleviates infraorbital nerve constriction induced thermal hyperalgesia in an operant assay. Direct application of botulinum toxin to dorsal root ganglia reversed the sciatic nerve entrapment induced decreases in withdrawal thresholds.