Analysis of axonal formation and extension from excitatory cortical neurons

为了阐明小鼠大脑皮层兴奋性神经元轴突的形成和发育，我们通过电穿孔标记脑室区细胞，并观察其后代的形态学变化。我们的观察表明，兴奋性皮层神经元与多极形状的中间区启动定向轴突生长之前，径向迁移到皮质板，这表明在这些细胞中的神经元极性从头建立从非极化阶段。这一过程似乎是兴奋性皮层神经元的一个共同特征，在以后的发育中表现出多种投射模式。

To clarify axonal formation and development of excitatory neurons in the mouse cerebral cortex, we labeled the ventricular zone cells by electroporation and examined morphological changes of their progeny. Our observation indicated that excitatory cortical neurons with multipolar shape in the intermediate zone initiate directed axon outgrowth before radial migration into the cortical plate, suggesting that neuronal polarity in these cells is established de novo from a nonpolarized stage. This process seems to be a common trait among excitatory cortical neurons, which show variety of projection patterns in later development.