Molecular mechanisms underlying axonal local protein synthesis in neurons

神经元轴突局部蛋白质合成的分子机制

• 批准号: 22500329
• 负责人: TAKEI Nobuyuki
• 金额: $ 2.75万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500329/
• 关键词: 細胞内情報伝達; 局所翻訳; 蛋白合成; 成長円錐; 翻訳調節; eIF2a; eIF2Be; 局所的翻訳; BDNF; Wnt3a; eEF2; GSK3b; eIF2Bε

Translation machinery in growth cone. Existence of ribosomes in growth cone was investigated by immunocytochemistry with anti-ribosomalprotein antibodies. Although it is widely believed that there is no ribosomes in axon, immunoreactivityagainst ribosomal proteins was recognized in growth cone of cultured dorsal root ganglia neurons and ofhippocampal neurons. In addition, several translation factors were found in growth cone of theseneurons. Precise morphological analysis of growth cone was performed by using atomic forcemicroscopy.Triggers of local translation in growth cone.Brain-derived neurotrophic factor (BDNF) and Wnt3a were identified as a trigger of local translation ingrowth cone. In addition to the [35S]methionin incorporation, puromycin-anti-puromycin immunocytochemistry method revealed that these factors activate novel protein synthesis in growth cones.These results indicate the existence of translational machinery in growth cone and strongly suggest thatlocal protein synthesis occurs in growth cones in response to certain growth factors.

生长锥中的翻译机械。使用抗核糖体蛋白抗体通过免疫细胞化学研究生长锥中核糖体的存在。尽管人们普遍认为轴突中不存在核糖体，但在培养的背根神经节神经元和海马神经元的生长锥中发现了针对核糖体蛋白的免疫反应性。此外，在这些神经元的生长锥中发现了几种翻译因子。使用原子力显微镜对生长锥进行精确的形态学分析。生长锥局部平移的触发因素。脑源性神经营养因子（BDNF）和Wnt3a被确定为生长锥局部平移的触发因素。除了[35S]蛋氨酸掺入之外，嘌呤霉素-抗嘌呤霉素免疫细胞化学方法揭示这些因子激活生长锥中的新型蛋白质合成。这些结果表明生长锥中存在翻译机制，并强烈表明生长锥中响应某些生长因子而发生局部蛋白质合成。

项目成果

PallidalHyperdopaminergic Innervation Induced by Neonatal Exposure To Epidermal Growth Factor; Implication in Schizophrenia

新生儿暴露于表皮生长因子引起的苍白球多巴胺能神经支配；

• 发表时间: 2012
• 作者: Nawa;Hiroyuki
• 通讯作者: Hiroyuki

神経細胞における翻訳調節:活動依存性と空間的特性

神经元的翻译调节：活动依赖性和空间特征

• 发表时间: 2011
• 作者: 武井延之;那波宏之
• 通讯作者: 那波宏之

ラット脊髄後根神経節細胞の成長円錐の神経栄養因子による構造変化

神经营养因子诱导大鼠脊髓背根神经节细胞生长锥的结构变化

• 发表时间: 2013
• 作者: Umahara T;Uchihara T;Yamada S;Hashimoto T;Akimoto J;Haraoka J;Iwamoto T;星治
• 通讯作者: 星治

神経細胞特異的翻訳調節機構

神经元特异性翻译调节机制

• 发表时间: 2012
• 作者: Yamanami-Irioka A;Uchihara T;Endo T;Irioka T;Watanabe M;Kitagawa M;Mizusawa H;武井延之監修
• 通讯作者: 武井延之監修

Protein Production of an Active Neurotrophic Factor, Neuregulin-1; Qualitative Comparison of In Vitro Translation Systems

活性神经营养因子 Neuregulin-1 的蛋白质生产；

• 发表时间: 2011
• 期刊: Neurosci Lett
• 影响因子: 2.5
• 作者: Wang R;Iwakura Y;Araki K;Sotoyama H;Takei N and Nawa H
• 通讯作者: Takei N and Nawa H