Involvement of Polycomb group genes in cell line property of human ES and iPS cells

Polycomb 组基因对人 ES 和 iPS 细胞细胞系特性的影响

• 批准号: 22500425
• 负责人: TOMOTSUNE Daihachiro
• 金额: $ 2.33万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500425/
• 关键词: ヒト ES 細胞; ヒトiPS 細胞; ポリコーム; 多能性幹細胞; 未分化維持機構; ヒトiPS細胞; ヒトES細胞; 未分化破綻; ポリコーム遺伝子; ES細胞; iPS細胞; 分化傾向; 未分化維持

In this study, we produced the spontaneous differentiation, and then analyzed global changes of gene expression during the spontaneous differentiation using DNA microarray. As results of the microarray analysis using four human pluripotent stem cell lines, we found that some genes were commonly up- or down-regulated during the collapse of undifferentiated state. Furthermore, we analyzed the role of polycomb group during the collapse of undifferentiated state by ChIP-seq and ChIP-QPCR.

本研究利用基因芯片技术，对自发分化过程中基因表达的整体变化进行了分析。作为使用四种人类多能干细胞系的微阵列分析的结果，我们发现在未分化状态的崩溃期间，一些基因通常上调或下调。此外，我们通过ChIP-seq和ChIP-QPCR分析了polycomb基团在未分化状态崩溃过程中的作用。

项目成果

GLOBAL ANALYSIS OF STABILITY OF UNDIFFERENTIATED STATE IN HUMAN PLURIPOTENT STEM CELLS

人类多能干细胞未分化状态稳定性的整体分析

• 发表时间: 2013
• 作者: Daihachiro Tomotsune;Akimi Mogi;Fengming Yue;Susumu Yoshie;Shoko Masuda;Ayana Sakurai;Daiki Fujita;Mika Morisaki-Nagai;Sakiko Takizawa-Shirasawa;Tadayuki Yokoyama;Katsunori Sasaki
• 通讯作者: Katsunori Sasaki

Fluid dynamic forces created by rotary orbital suspension culture promote cardiomyogenic differentiation of human embryonic stem cells.

旋转轨道悬浮培养产生的流体动力促进人胚胎干细胞的心肌分化。

• DOI: 10.5405/jmbe.1356
• 发表时间: 2013
• 期刊: J. Med. Biol. Eng
• 作者: Akimi Mogi;Shunsuke Takei;Hisashi Shimizu;Haruki Miura;Daihachiro Tomotsune*;and Katsunori Sasaki
• 通讯作者: and Katsunori Sasaki