Effect of AMPK activation on mechanisms responsible for exercise-induced expression of monocarboxylate transporter

AMPK 激活对运动诱导单羧酸转运蛋白表达机制的影响

• 批准号: 22500616
• 负责人: HAMADA Taku
• 金额: $ 2.75万
• 依托单位: Osaka University of Health and Sport Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500616/
• 关键词: 運動; MCT; AMPK; AICAR; caffeine; 一過性運動; 骨格筋; CD147; 運動強度; 高強度短時間運動; モノカルボン酸輸送担体; AMPキナーゼ; 乳酸

The purpose of this study was to examine the hypothesis that signaling molecules AMPKwould mediate the acute exercise-induced expression of monocarboxylate transporters MCT1and MCT4 in skeletal muscle. The male SD rats were subjected to a bout of high-intensityintermittent swimming (HIS) or low-intensity prolonged swimming exercise (LIS). AMPKphosphorylation was increased in response to LIS and HIS in exercised muscle. MCT1 andMCT4 mRNA were transiently upregulated by HIS and LIS. MCT1 protein was increased afterLIS, but MCT4 protein was increased after HIS, while no changes in MCT1 protein wereobserved. Direct exposure of epitrochlearis to 0.5mM 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) or 1mM caffeine increased both MCT1 and MCT4 mRNA but notMCT1 and MCT4 protein. When 1mM caffeine-induced pAMPK was inhibited by dantrolene,neither MCT1 nor MCT4 mRNA was increased. The present findings suggest that acute exercisemay upregulate MCT1 and MCT4 mRNA expression thrg-mediated pathway in skeletalmuscle.

这项研究的目的是检查信号分子AMPK的假设将介导骨骼肌中急性运动诱导的单羧酸盐转运蛋白MCT1和MCT4的表达。雄性SD大鼠进行了一场高强度的间歇性游泳（他）或低强度的长时间游泳运动（LIS）。响应于LIS及其运动肌肉的响应于Ampkphosphylation。 MCT1和MCT4 mRNA通过其和LIS瞬时更新。 MCT1蛋白增加了后LIS，但是MCT4蛋白在其后增加，而MCT1蛋白的变化没有变化。将表质核糖直接暴露于0.5mm 5-氨基咪唑-4-羧化酰胺-1-β-D-核呋喃糖苷（AICAR）或1mm咖啡因会增加MCT1和MCT4 mRNA，但NOTMCT1和MCT4蛋白会增加。当Dantrolene抑制1mm咖啡因诱导的PAMPK时，MCT1和MCT4 mRNA均未增加。目前的发现表明，急性锻炼上调了骨骼肌肉中的MCT1和MCT4 mRNA表达TRHG介导的途径。

项目成果

カフェインによる IKK/NFκB シグナル泯性化を介した骨格筋インスリンシグナル伝達抑制作用.

咖啡因通过增强 IKK/NFκB 信号传导对骨骼肌胰岛素信号传导产生抑制作用。

• 发表时间: 2012
• 作者: 江川達郎;〓田諭志;大島里詠子;浜田 拓;林達也
• 通讯作者: 林達也

Regulation of the acute exercise-induced expression of the monocarboxylate transporters MCT1 and MCT4 in skeletal muscle.

骨骼肌中单羧酸转运蛋白 MCT1 和 MCT4 急性运动诱导表达的调节。

• 发表时间: 2013
• 期刊: J. Phys. Fitness Sports Med.
• 作者: Hamada T; Takimoto M.
• 通讯作者: Takimoto M.

Effect of AMPK activation on acuteexercise-induced monocarboxylatetransporter (MCT) 1 and MCT4 mRNAexpression in skeletal muscle.

AMPK 激活对骨骼肌中急性运动诱导的单羧酸转运蛋白 (MCT) 1 和 MCT4 mRNA 表达的影响。

• 发表时间: 2012
• 作者: Takimoto M;Takeyama M;Enoki T;HamadaT.
• 通讯作者: HamadaT.

Regulation of the acute exercise-induced expression of the monocarboxylate transporters MCT1 and MCT4 in skeletal muscle

骨骼肌中单羧酸转运蛋白 MCT1 和 MCT4 急性运动诱导表达的调节

• 发表时间: 2013
• 期刊: J. Phys. Fitness Sports Med.
• 作者: Hamada T;Takimoto T
• 通讯作者: Takimoto T

Caffeine acutely modulates signaling mechanisms of glucose transport and protein synthesis in rat skeletal muscle

咖啡因急性调节大鼠骨骼肌中葡萄糖转运和蛋白质合成的信号机制

• 发表时间: 2011
• 期刊: Sportology
• 作者: Tatsuro Egawa;et al
• 通讯作者: et al