Establishment of stable and effective methods for formation of indigo from various naturalindigo plants andeffective utilization of remaining coloring matters after the dyeing with conventional methods.

从不同处理方法处理的天然青黛植物叶片中含青蓝的量来考察促进水解酶活性和促进氧化偶联反应的方法的有效性。结果表明，用烘干机烘干、手工揉搓、打浆等操作，可使天然青黛叶片中的蓝宝石含量增加。其次，研究了用压碎的干叶直接染色是否可以代替沉淀法和数库墨法染色。结果证明，这种简化的染色方法是非常有效的。从天然青黛植物中制备沉淀青蓝后的残留物被证明适用于堆肥或Sukumo的制备。

Effectiveness of the methods for promotion of the activation of hydrolase andfor promotion of theoxidative coupling reaction was examined from the amount of indigo included in the leaves of natural indigo plants treated with various means. It was proved that the operations as the dry with a drier, the rubbing together by hand and the beating lead to an increase in indigo in the leaves of natural indigo plants. Next, it was examined whether the direct dyeing by using a crushed dry leaf of naturalindigo plants ispossible instead of thedyeingwith precipitation indigo or sukumo indigo. As a result, it has been confirmed that this simplified method of dyeing was very effective.The residues after the preparation of precipitation indigo from natural indigo plants proved to be applicable to the compost or the preparation of sukumo.