A general survey for teaching-aid kit design of biological teaching-materials and their apparatus by sterile condition

无菌条件下生物教材及其器具教具设计概况

• 批准号: 22500805
• 负责人: MAEDA Masuo
• 金额: $ 1.91万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500805/
• 关键词: 科学教育; 植物生殖現象; 教材キット; シダ・裸子植物; ミズワラビ・カニクサ; イチョウ; 顕微鏡; 無菌技術; シダ植物; ミズワラビ; カニクサ; 裸子植物イチョウ; 無菌培養

A general survey for design of biological teaching-materials and their apparatus were especially experimented on reproduction process in plant. Subsequently, an in vitro kit was assembled and designed for biological teaching. Two species of fern (Ceratopteris richardii,and Lygodoium japonicum Sw.)were used for in vitro culture. The spores sterilized with routine techniques and subsequently desiccated spores were settled with pre-sterilized 1/2 Murashige and Skoog medium’s salts into the polystyrene vessel installed with ED-sterilized syringe filter on top portion of the vessel. The developing gametophyte (archegonium, antheridium, and sperm) can easily observe attaching the bottom of culture vessel with a magnifier or binocular. Sperm of Ginkgo bilobawere skillfully observed with hanging-drop culture method of pollen tube (spermatocyte) derived from young female seeds.

对生物教材的设计进行了概览，并着重对植物生殖过程进行了实验。随后，组装并设计了用于生物教学的体外试剂盒。以两种蕨类植物（角蕨和日本羊尾草）进行离体培养。用常规技术灭菌的孢子，随后用预灭菌的1/2 Murashige和Skoog培养基的盐将干燥的孢子沉淀到聚苯乙烯容器中，容器顶部安装有ed灭菌的注射器过滤器。利用放大镜或双筒望远镜可以很容易地观察到附着在培养容器底部的配子体（卵原体、精子和精子）的发育过程。用年轻雌种子花粉管（精母细胞）悬垂培养法对银杏二叶精子进行了观察。

项目成果

自動車内ディスプレイとしてのインビトロ植物の可能性に関する研究

体外植物作为车载显示器的潜力研究

• 发表时间: 2011
• 期刊: 福井大学教育地域科学部紀要
• 作者: 佐々木由希、高城啓一、石川和彦;前田桝夫、奥野信一
• 通讯作者: 前田桝夫、奥野信一