Strategy for overcoming drug resistance of leukemic stem cells by using micro-RNA targeted for telomerase

本实验的目的是了解microRNA对端粒酶表达的调控作用。结合耐药细胞的甲基化分析结果，我们试图开发克服癌症干细胞耐药性的治疗策略。结果表明，端粒酶和MDR 1基因启动子甲基化水平在耐药过程中保持稳定。相反，观察到端粒酶和MDR 1蛋白的表达增加，这是通过转录因子STAT 5的激活引起的。

The object of our experiment is to know microRNA regulating telomerase expression. Together with the results of methylation analysis of drug resistant cells, we tried to develop therapeutic strategies for overcoming drug resistance of cancer stem cells. As a result, methylation level of both telomerase and MDR1 promoter lesion was stable during acquisition of drug resistance. Instead, increased expression of telomerase and MDR1 protein which was caused through the activation of transcription factor STAT5, was observed.