Analysis of regulation mechanism for metabolic pathway based on comparative proteomics for protein abundance

基于蛋白质丰度比较蛋白质组学分析代谢途径调控机制

• 批准号: 22510207
• 负责人: KITO Keiji
• 金额: $ 2.41万
• 依托单位: Meiji University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22510207/
• 关键词: 比較プロテオミクス; 代謝経路; 酵母; 質量分析; 出芽酵母; 絶対量計測; PCS-MS法

In this study, we analyzed proteome expression pattern for multiple yeast species grown on different culture conditions to understand proteome differences involved in interspecies varieties. Eight yeast species grew with comparable growth rate on glucose medium. In contrast, four species of Saccharomyces genus, including S. cerevisiae, showed slow growth rate by two-to three-fold on glycerol and acetate medium, compared to four species belonging to different genus. Some groups of proteins involved in mitochondrial metabolic pathway and response to oxidative stress showed different abundance between Saccharomyces and other genus when grown on glycerol. It is suggested that such differences in abundance of proteins with specific functions could be responsible for disparity of growth rate among yeast species.

在这项研究中，我们分析了蛋白质组表达模式的多种酵母菌生长在不同的培养条件下，以了解蛋白质组的差异涉及种间品种。八种酵母菌在葡萄糖培养基上以相当的生长速率生长。与此相反，包括S.在甘油和醋酸盐培养基上，酿酒酵母的生长速度比属于不同属的四个种慢2 - 3倍。在甘油培养基中，酵母属和其他属的线粒体代谢途径和氧化应激反应相关蛋白质组的丰度存在差异。这表明，具有特定功能的蛋白质丰度的这种差异可能是负责酵母物种之间的生长速度的差距。

项目成果

Hierarchical Use of Peptide-concatenated Standard (PCS) for Absolute Protein Quantification with Mass Spectrometry.

肽连接标准品 (PCS) 的分层使用用于质谱绝对蛋白质定量。

• 发表时间: 2010
• 作者: Keiji Kito;Takashi Ito
• 通讯作者: Takashi Ito

Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins

• DOI: 10.1002/pmic.200900497
• 发表时间: 2010-01-01
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Kato, Mitsunori;Kito, Keiji;Ito, Takashi
• 通讯作者: Ito, Takashi

酵母種間における比較プロテオミクス

酵母物种之间的比较蛋白质组学

• 发表时间: 2012
• 作者: 大西美帆子;伊藤遼;長谷川勇輔;正岡昌一郎;紀藤圭治
• 通讯作者: 紀藤圭治

酵母種間における代謝経路の比較プロテオミクス

酵母物种代谢途径的比较蛋白质组学

• 发表时间: 2012
• 作者: 大西美帆子;伊藤遼;長谷川勇輔;正岡昌一郎;紀藤圭治;紀藤圭治
• 通讯作者: 紀藤圭治