Analytical Chemical Study on the Mechanism of Photosynthetic Oxygen Evolution

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To elucidate the energetics driving water oxidation by Photosystem II, in which redox properties of cofactors were obscure because of insufficient measurement technique, redox potentials of two key cofactors, the primary electron acceptor Pheophytin aand the primary quinone QA, were determined by spectroelectrochemistry using an optically-transparent thin-layer electrode cell. On the basis of the results of site-directed mutants and ion-exchanged PSII preparations, the redox potential of the primary electron donor P680, which is the source power for the water oxidation, and relationship between the oxygen-evolution activity and the cofactors' redox properties were elucidated.