New practical technology using pulsed power - material incorporation for fertilized eggs with a high intensity voltage with very short pulse duration

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To establish a simple and efficient method to introduce materials into living organisms, pulse system was designed and tested. This system consists of a pulsed power generator to generate a high intensity voltage with very short pulse duration, several RC discharge circuits to generate low voltage pulses with long pulse duration, a trigger signal generator, and reactor. By using this system, chemical substances, such as cycloheximide and actinomycin D, or a fluorescent dye had been incorporated into fertilized Medaka eggs without damage inside them.