Functional analysis of thioredoxin family proteins in higher plant chloroplasts

高等植物叶绿体的氧化还原状态在光照和黑暗条件下波动很大。根据氧化还原状态，硫氧还蛋白(Trx)通过减少蛋白质的二硫键来调节蛋白质的功能。在拟南芥中，质基质Trx-异构体由10个成员组成，分为5种类型。F型和m型Trxs调节主要参与初级代谢的酶的活性，包括Calvin循环，而x型、y型和z型Trxs与氧化应激反应有关。然而，缺乏证明其生理重要性的遗传学证据。我们制备了针对每种Trx亚型的特异性抗体，并测定了它们的浓度。叶绿体基质中的主要Trx蛋白为m型Trx，有4种异构体。我们分析了一个拟南芥trx m突变体。

The redox state of higher plant chloroplasts fluctuates widely under light and dark conditions. Depending on the redox state, thioredoxins (Trx) regulate the function of proteins by reducing their disulfide bridge. In Arabidopsis, plastidial stroma Trx-isoforms comprise ten members, into five types. The f- and m-type Trxs regulate the activity of enzymes mainly involved in the primary metabolism, including the Calvin cycles, while x-, y- and z-type Trxs are related with oxidative stress responses. However, the genetic evidence for its physiological importance is lacking. We prepared specific antibodies for each Trx isoforms and determined their concentration. The m-type Trx with four isoforms was major Trx proteins in the chloroplast stroma. We analyzed an Arabidopsis trx m mutant.