Analyses on the functions of chloroplast actin filaments

叶绿体对环境光条件的响应在细胞内重新定位。叶绿体的光迁移反应一般是由蓝光受体--趋光素介导的。我们已经在拟南芥细胞中表明，叶绿体的运动利用叶绿体肌动蛋白短丝(cp-肌动蛋白细丝)，它不同于细胞质肌动蛋白细丝，是叶绿体所特有的。在这项工作中，我们发现了新的cp-肌动蛋白细丝介导的叶绿体运动机制，在地钱和苔藓细胞中也发现了这一机制，这两种植物被认为是第一批陆地植物，在进化上与拟南芥相距较远。在地钱和苔藓细胞中，叶绿体的光重新定位也受光敏素以外的受体的调节，并对这些反应中的cp-肌动蛋白动态进行了分析。

Chloroplasts relocate in the cell in response to environmental light condition. The chloroplast photorelocation response is generally mediated by the blue light receptor, phototropin. We have been shown in Arabidopsis cells that the chloroplast movement utilizes short chloroplast actin filaments (cp-actin filaments), which are different from cytoplasmic actin filaments and unique to chloroplast. In this work, we showed that the novel cp-actin filaments-mediated movement mechanism of chloroplasts are also found in cells of liverwort and moss which are considered to be the first land plants and evolutionally distant from Arabidopsis. In liverwort and moss cells, chloroplast photorelocation are also regulated by receptors other than phototropin, cp-actin dynamics are analyzed in these responses.