Exploring evolution and figure of early fungal clade "LKM11"in oxygen-depleted environment

探讨早期真菌进化枝“LKM11”在缺氧环境中的演化和形态

• 批准号: 22570105
• 负责人: NAGAHAMA Takahiko
• 金额: $ 2.91万
• 依托单位: Higashichikushi Junior College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570105/
• 关键词: 菌類; LKM11 クレード; 初期進化; LKM11クレード

For exploring early evolution of fungi, we investigated fungaldiversity in sediments colleted in eight lakes in west Japan. By using culture-independentmethod with fungal-specific PCR of SSU rDNA, we revealed an large extent of novel fungaldiversity in lacustrine environment. Four hundreds fungal phylotypes were obtained fromPCR clone libraries of six lakes successfully amplified. Novel (<97% identity) phylotypesare 213 (53.3%) in all fungal phylotypes. There were 187 phylotypes (46.8%) related toDikarya (Ascomycota + Basidiomycota). Whereas, Non-Dikarya fungi (Chytridiomycota +Blastocladiomycota + LKM11 etc) were 53.3%, 74.8% of which were estimated to be novelphylotypes with <97% affinities. All LKM11 phylotypes were novel. Early fungal lineagesoccupied a majority of all fungal phylotypes from the lacustrine environment in west Japanand indicated an unexpected diversity and novelty.

为了探索真菌的早期进化，我们调查了日本西部八个湖泊沉积物中的真菌多样性。通过对SSU rDNA进行真菌特异性PCR的非培养方法，我们发现了湖泊环境中大量新的真菌多样性。从六湖的PCR克隆文库中成功扩增出400个真菌分型。在所有的真菌分类系统中，新分类系统有213个（53.3%）。与双核菌门（子囊菌门+担子菌门）有关的真菌187种（46.8%）。非双核真菌（壶菌门+芽枝菌门+LKM 11等）占53.3%，其中74.8%为新种，亲缘关系<97%。所有LKM 11突变类型均为新类型。早期真菌Lineagesoccupied大多数的真菌真菌类群从湖泊环境在日本西部，并表明了意想不到的多样性和新奇。

项目成果

Fungal Diversity in Deep-Sea Environments In Biology of Marine Fungi,Progress in Molecular and Subcellular Biology,Edited by C. Raghukumar

海洋真菌生物学中深海环境中的真菌多样性，分子和亚细胞生物学进展，C. Raghukumar 编辑

• 发表时间: 2012
• 作者: Nagahama;T. & Nagano;Y. Cultured and Uncultured
• 通讯作者: Y. Cultured and Uncultured

Fungal diversity in deep-sea sediments - the presence of novel fungal groups

• DOI: 10.1016/j.funeco.2010.01.002
• 发表时间: 2010-11-01
• 期刊: FUNGAL ECOLOGY
• 影响因子: 2.9
• 作者: Nagano, Yuriko;Nagahama, Takahiko;Horikoshi, Koki
• 通讯作者: Horikoshi, Koki

Biosurfactant-producing yeast isolated from Calyptogena soyoae (Deep-Sea Cold-Seep Clam) in the deep sea

从深海中的Calyptogena soyoae（深海冷浸蛤）中分离出产生生物表面活性剂的酵母

• 发表时间: 2010
• 期刊: Journal of Bioscience and Bioengineering 110(2)
• 作者: Konishi M.;Fukuoka T.;Nagahama T.;Morita T.;Imura T.;Kitamoto D;Hatada Y.
• 通讯作者: Hatada Y.

Halosarpheia japonica sp. nov. (Halosphaeriales, Ascomycota) from marine habitats in Japan

• DOI: 10.1007/s11557-010-0731-0
• 发表时间: 2012-02
• 期刊: Mycological Progress
• 影响因子: 2.4
• 作者: M. Abdel-Wahab;Takahiko Nagahama

深海環境における真菌多様性

深海环境中的真菌多样性

• 发表时间: 2022
• 作者: Harutaka Hata;Keita Koeda;Masahiro Aizawa;Kazuo Sakamoto;Rei Ueshima;長野由梨子
• 通讯作者: 長野由梨子