Elucidation of the mechanism of receptor activation by ENF peptide family

ENF多肽是一类昆虫细胞因子，具有多种生物活性，如调节幼虫生长、细胞增殖、刺激免疫细胞等。在本研究中，我们利用甲醇营养酵母巴斯德毕赤酵母构建了ENF多肽的表达系统，该酵母是高效表达外源蛋白的宿主。为了抑制酶的降解，我们开发了与硫氧还蛋白融合的多肽的制备方法，并成功地生产出了高效的ENF多肽。此外，我们还研究了ENF多肽与GBP-BP之间的相互作用，这与ENF多肽活性的终止有关。

ENF peptides are insect cytokines that possesses diverse biological activities such as larval growth regulation, cell proliferation, and stimulation of immune cells. In the present study, we constructed the expression systems of ENF peptides using the methylotrophic yeast Pichia pastoris, which is an efficient host for high-level production of heterologous proteins. To inhibit degradation by protease, we developed the method to produce peptides fused to thioredoxin and we successfully produced ENF peptides efficiently. In addition, we studied the interactions between ENF peptides and GBP-BP that is related to the end of the activation of ENF peptides.