Elucidation of dynamic structural changes of protein antigens and antibodies in their interactions

阐明蛋白质抗原和抗体在相互作用中的动态结构变化

• 批准号: 22570121
• 负责人: ODA Masayuki
• 金额: $ 3.08万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570121/
• 关键词: 分子認識; 生体分子; タンパク質; 分析化学; 免疫学; 蛋白質

Antibodies recognize various types of antigens, different shapes and sizes, and are good targets for the analysis to elucidate the structural dynamics of proteins. In this study, I changed the structures of protein antigens by chemical modification and disulfide-bonddeletion, and analyzed how the antibodies could recognize the slight and dynamic differences in antigens using the methods of binding kinetics and thermodynamics. As a result, I could detect the plural states of protein antigens and antibodies in solution, and quantitatively determine the effects of protein fluctuations.

抗体识别各种类型的抗原，不同的形状和大小，并且是用于分析以阐明蛋白质的结构动力学的良好靶标。在本研究中，我通过化学修饰和二硫键缺失来改变蛋白质抗原的结构，并利用结合动力学和热力学方法分析抗体如何识别抗原的微小动态差异。结果，我可以检测溶液中蛋白质抗原和抗体的多种状态，并定量确定蛋白质波动的影响。

项目成果

Correlations between the Dynamics of Antibody-Antigen Complexes and the Antigen Binding Thermodynamics of Antibodies

抗体-抗原复合物动力学与抗体抗原结合热力学之间的相关性

• 发表时间: 2010
• 作者: Shingo Shimoyama;Yuuki Asano;Kyohei Takahashi;Kazumi Saikusa;Aritaka Nagadoi;Hiroaki Tachiwana;Hitoshi Kurumizaka;Yoshifumi Nishimura;Satoko Akashi;明石知子;織田 昌幸;織田昌幸;明石知子;Masayuki Oda
• 通讯作者: Masayuki Oda

Generation of single-chain Fv of anti-(4-hydroxy-3-nitrophenyl)acetyl antibody and its heteroclitic antigen binding

抗(4-羟基-3-硝基苯基)乙酰基抗体单链Fv的产生及其异粘附抗原结合

• 发表时间: 2011
• 作者: 七種和美、明石知子;他6名;Yusuke Tanaka
• 通讯作者: Yusuke Tanaka

蛋白質の天然構造と変性構造を見分ける抗体の識別能

抗体区分天然和变性蛋白质结构的辨别能力

• 发表时间: 2011
• 作者: 七種和美、明石知子;他6名;Yusuke Tanaka;明石知子;佐野 智生
• 通讯作者: 佐野 智生

Effects of substrate strain on binding thermodynamics and kinetics of catalytic antibodies

底物菌株对催化抗体结合热力学和动力学的影响

• 发表时间: 2011
• 作者: 七種和美、明石知子;他6名;Yusuke Tanaka;明石知子;佐野 智生;明石知子;Masayuki Oda
• 通讯作者: Masayuki Oda

Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form

• DOI: 10.1016/j.abb.2009.11.024
• 发表时间: 2010-02-15
• 期刊: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
• 影响因子: 3.9
• 作者: Oda, Masayuki;Kitai, Aki;Azuma, Takachika
• 通讯作者: Azuma, Takachika