Elucidation of dynamic structural changes of protein antigens and antibodies in their interactions

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Antibodies recognize various types of antigens, different shapes and sizes, and are good targets for the analysis to elucidate the structural dynamics of proteins. In this study, I changed the structures of protein antigens by chemical modification and disulfide-bonddeletion, and analyzed how the antibodies could recognize the slight and dynamic differences in antigens using the methods of binding kinetics and thermodynamics. As a result, I could detect the plural states of protein antigens and antibodies in solution, and quantitatively determine the effects of protein fluctuations.