Elucidation of the reaction mechanisms for mitochondrial respiratory complex I

为了阐明位于线粒体呼吸链入口处的巨大复合体I的作用机制，确定整个复合体I的原子结构是必不可少的。我们建立了牛心完整复合体I的纯化方法，并考察了二维和三维结晶条件，提供了酶复合体的结晶。测定了纯化酶中FMN的共振拉曼光谱，表明存在一种防止活性氧产生的机制。

For elucidation of the mechanisms of huge Complex I, locates at the entrance of the mitochondrial respiratory chains, determination of the atomic structure of the whole Complex I is essential. We have established the method for purification of intact Complex I from bovine heart and surveyed the 2-D and 3-D crystallization conditions to provide the crystals of the enzyme complex. The resonance Raman spectra of FMN in purified enzyme were determined, suggest the existence of a mechanism for prevention of spontaneous production of reactive oxygen species.