Analysis of physiological role of SVIL for cytokinesis

我们发现，超绒毛蛋白（SVIL）定位于中央纺锤体在胞质分裂。这种定位依赖于SVIL的Ser 238的磷酸化。SVIL-238 A（Ser 238）替换为丙氨酸后的表达抑制了胞质分裂的进程。此外，在SVIL-S238 A表达细胞中观察到细胞动力学沟的异常进入。我们的研究结果表明，SVIL的磷酸化是必不可少的胞质分裂的完成。

We found that Supervillin (SVIL) was localized to the central spindle during cytokinesis. The localization was dependent on the phosphorylation of Ser238 of SVIL. Expression ofSVIL with the substitution of Ser238 (SVIL-S238A) to alanine inhibited progression of cytokinesis. Furthermore, aberrant ingression of cytokinetic furrow was observed in SVIL-S238A expressing cells. Our results show that phosphorylation of SVIL is essential for the completion of cytokinesis.