Analysis of retinotectal projection and tectal layer formation

视网膜顶盖投影和顶盖层形成分析

• 批准号: 22570199
• 负责人: NAKAMURA Harukazu
• 金额: $ 3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570199/
• 关键词: 網膜視蓋投射; 視蓋層形成; ニワトリ胚; エレクトロポレーション; Engrailed; 視蓋; 層形成; Tet-on; En2; スライスカルチャー; 動画; 層構築; Er81

In this study, we have shown that some part of the optic fibers run deep layers of the tectum from the first. These fibers are transient and disappear after birth. En2 is expressed in laminae g-j of SGFS in tecta of around E10. Misexpression of En2 showed that En2 expressing cells cannot stay in the superficial layers of the tectum. Time lapse analysis showed that En2 expressing cells went out of the superficial layer and settled in lamina i. The results indicate that En2 plays crucial roles in tectal layer formation. We found that layer IV, and V are composed of cells that have migrated tangentially, and that neuropilin1 is expressed in layer IV, V cells and sema3A is expressed in layer VI cells. Sema3A overexpression resulted in abnormal layer formation of the tectum. The results indicate that neuropilin-Semaphorin repulsive system plays crucial roles in tectal layer formation.

在这项研究中，我们已经表明，部分光纤从第一个顶盖的深层开始。这些纤维是短暂的，出生后就消失了。En2在SGFS的g-j层中表达，表达量约为E10。En2的错表达表明，表达En2的细胞不能停留在顶盖的表层。延时分析显示，表达En2的细胞从浅层进入i层，表明En2在顶层形成过程中起着至关重要的作用。我们发现IV层、V层由切向迁移的细胞组成，neuropilin1在IV层、V层细胞中表达，sema3A在VI层细胞中表达。Sema3A过表达导致顶盖层形成异常。结果表明，神经肽-信号素排斥系统在顶层形成中起着至关重要的作用。

项目成果

Conditional knockdown of target gene expression: Tet-on of transcription of double strand RNA. Chick 7: Avian Model Systems

目标基因表达的条件性敲低：双链 RNA 转录的 Tet-on。

• 发表时间: 2012
• 作者: Hou;X.;Omi;M.;Harada;H.;Ishii;S.;Takahashi;Y.;Nakamura;H.
• 通讯作者: H.

Involvement of Mitf in the retinal pigment epthelium development and its possible regulators

Mitf参与视网膜色素上皮发育及其可能的调节因子

• 发表时间: 2010
• 作者: Daisuke Nishihara;Nagahara Tsukiji;Ichiro Yajima;Kazuhisa Takeda;Shigeki Shibahara;Harukazu Nakamura;Hiroaki Yamamoto
• 通讯作者: Hiroaki Yamamoto

Organizing signal for development of the tectum and cerebellum. UCL-Tohoku University Symposium, From Cell/Developmental Biology to Neuroscience

组织顶盖和小脑发育的信号。

• 发表时间: 2010
• 作者: 本村恵理子;成田知弘;西松伸一郎;坂井雅夫;Harukazu Nakamura
• 通讯作者: Harukazu Nakamura

UCL-Tohoku University Symposium

UCL-东北大学研讨会

• 发表时间: 2010
• 作者: 永井晶子;菊池摩仁;大森紹仁;近藤真理子;Harukazu Nakamura & Shinichi Ohnuma
• 通讯作者: Harukazu Nakamura & Shinichi Ohnuma

Novel retinotectal projection pathway in deeper laminae of the developing chick optictectum

发育中的雏鸡视顶盖更深层中的新型视网膜顶盖投射途径

• 发表时间: 2011
• 作者: Minoru Omi;Hidekiyo Harada;Harukazu Nakamura
• 通讯作者: Harukazu Nakamura