Analysis of retinotectal projection and tectal layer formation

在这项研究中，我们已经表明，一些部分的光纤运行从第一顶盖深层。这些纤维是短暂的，在出生后消失。En 2在E10左右的覆盖层中的SGFS的层g-j中表达。En 2的错误表达表明表达En 2的细胞不能停留在顶盖的浅层。时间推移分析显示，表达En 2的细胞走出浅层并定居在板层i。结果表明，En 2在顶盖层的形成中起着至关重要的作用。我们发现，第四层，和V是由切向迁移的细胞，和neuropilin 1表达在第四层，V细胞和sema 3A表达在第VI层细胞。Sema 3A过表达导致顶盖的异常层形成。结果表明，神经毡蛋白-脑信号蛋白排斥系统在顶盖层的形成中起重要作用。

In this study, we have shown that some part of the optic fibers run deep layers of the tectum from the first. These fibers are transient and disappear after birth. En2 is expressed in laminae g-j of SGFS in tecta of around E10. Misexpression of En2 showed that En2 expressing cells cannot stay in the superficial layers of the tectum. Time lapse analysis showed that En2 expressing cells went out of the superficial layer and settled in lamina i. The results indicate that En2 plays crucial roles in tectal layer formation. We found that layer IV, and V are composed of cells that have migrated tangentially, and that neuropilin1 is expressed in layer IV, V cells and sema3A is expressed in layer VI cells. Sema3A overexpression resulted in abnormal layer formation of the tectum. The results indicate that neuropilin-Semaphorin repulsive system plays crucial roles in tectal layer formation.