Analysis of retinotectal projection and tectal layer formation

在这项研究中，我们已经证明，从第一次开始，某些部分的光纤穿过顶盖的深层。这些纤维是暂时的，出生后就消失了。EN2在约E10的顶盖中表达于SGFS的G-j板层。EN2的错误表达表明，表达EN2的细胞不能停留在顶盖的浅层。时间推移分析显示，EN2表达的细胞从浅层走出，并在I层沉积。结果表明，EN2在顶盖层的形成中起关键作用。我们发现，第IV层和第V层是由切线迁移的细胞组成的，在第IV层、第V层细胞和在第VI层细胞中表达了神经粘连蛋白1和Sema3A。Sema3A过度表达导致顶盖异常成层。结果表明，神经匹林-分指排斥素系统在顶盖层的形成过程中起着重要作用。

In this study, we have shown that some part of the optic fibers run deep layers of the tectum from the first. These fibers are transient and disappear after birth. En2 is expressed in laminae g-j of SGFS in tecta of around E10. Misexpression of En2 showed that En2 expressing cells cannot stay in the superficial layers of the tectum. Time lapse analysis showed that En2 expressing cells went out of the superficial layer and settled in lamina i. The results indicate that En2 plays crucial roles in tectal layer formation. We found that layer IV, and V are composed of cells that have migrated tangentially, and that neuropilin1 is expressed in layer IV, V cells and sema3A is expressed in layer VI cells. Sema3A overexpression resulted in abnormal layer formation of the tectum. The results indicate that neuropilin-Semaphorin repulsive system plays crucial roles in tectal layer formation.