Genetic analysis of the haploid strain in the potato late blight pathogen Phytophthora infestans

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Although the potato late blight pathogen, Phytophthora infestans, has long been believed to be diploid or of higher level of ploidy, we discovered a haploid isolate recently. In this study, we carried out genetic analyses on this isolate, including (1)molecular phylogenetic analysis based on nuclear and mitochondrial genes; (2) flow cytometric analysis of nuclear DNA content and stability of genome; (3)microscopic observation on the somatic nuclei and chromosomes; (4)growth characteristics; (5)production of auxotrophic mutants; (6)establishment of transformation method.