Genetic analysis of the haploid strain in the potato late blight pathogen Phytophthora infestans

马铃薯晚疫病致病菌致病疫霉单倍体菌株的遗传分析

• 批准号: 22580050
• 负责人: TAGA Masatoki
• 金额: $ 2.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580050/
• 关键词: 疫病菌; ジャガイモ・ゲノム; 倍数性; Phytophthora; 遺伝学; 半数体; 染色体; ゲノム; 形質転換; nit変異; ジャガイモ

Although the potato late blight pathogen, Phytophthora infestans, has long been believed to be diploid or of higher level of ploidy, we discovered a haploid isolate recently. In this study, we carried out genetic analyses on this isolate, including (1)molecular phylogenetic analysis based on nuclear and mitochondrial genes; (2) flow cytometric analysis of nuclear DNA content and stability of genome; (3)microscopic observation on the somatic nuclei and chromosomes; (4)growth characteristics; (5)production of auxotrophic mutants; (6)establishment of transformation method.

虽然马铃薯晚疫病菌一直被认为是二倍体或倍性较高的分离物，但我们最近发现了一个单倍体分离物。本研究对该菌株进行了遗传分析，包括(1)基于核基因和线粒体基因的分子系统发育分析；(2)核DNA含量和基因组稳定性的流式细胞仪分析；(3)体细胞核和染色体的显微观察；(4)生长特性；(5)营养缺陷型突变体的产生；(6)转化方法的建立。