Immunoelectron microscopy using aldehyde fixation with oxygen- bubbling for highly preserving protein in pathogen-infected anduninfected plant tissues

使用醛固定和氧气鼓泡的免疫电子显微镜可高度保存病原体感染和未感染植物组织中的蛋白质

• 批准号: 22580048
• 负责人: 朴 杓允
• 金额: $ 2.75万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580048/
• 关键词: 酸素バブリング; 感染応答タンパク; 糖タンパク; グルタルアルデヒド; 微細構造; 冠根; トルイジン青; 液胞; 酸素バブリング固定; グルタルアルデヒド固定; 根冠; 感染応答蛋白質; 糖蛋白質; トルイジン

A new chemical fixation method was developed successfully in this project. On fixing plants with glutaraldehyde together with oxygen bubbling, the higher preservation of glycoproteins was achieved, resulting in observing fine ultrastructure under electron microscope. The cell images involving membranes and matrix of organelles, cytoplasm and cell components were excellent. However, it was not yet apparent whether the bubbling fixation was good enough to the preservation of protein for immunoelectron microscopy or not.

本课题成功开发了一种新的化学固定方法。戊二醛固定结合氧气鼓泡固定，糖蛋白保存率高，电镜下观察到细微的超微结构。细胞图像包括细胞器的膜和基质、细胞质和细胞成分。然而，鼓泡固定是否足以保存免疫电镜所需的蛋白质，目前尚不清楚。

项目成果

Zinc compounds potentiated mycovirus transmission by attenuation of heterogenic incompatibility in Rosellinia necatrix.

锌化合物通过减弱 Rosellinia necatrix 中的异源不相容性来增强真菌病毒的传播。

• 发表时间: 2013
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: Ikeda;K.;Inoue;K.;Kida,C.;Uwamori,T.;Sasaki;A.;Kanematsu;S.;Park;P
• 通讯作者: P

細胞を読む(活性酸素とカビ病原性)

解读细胞（活性氧和真菌致病性）

• 发表时间: 2012
• 作者: 大西浩平;張勇;曵地康史;加賀谷奏，山下和宏，高橋正和，亀井誠之，一石昭彦，藤村 真;朴杓允
• 通讯作者: 朴杓允

Genetic analysis on mycelial incompatibility in Rosellinia necatrix

洛神花菌丝体不亲和性的遗传分析

• 发表时间: 2012
• 作者: Ikeda,K.,Inoue,K.,Nakamura,H.,Kanematsu,S.;Park,P
• 通讯作者: Park,P

Comparative study of ultrathin section shrinkage in various epoxy blocks:Sectioning from Spurr’s block causes shrinkage in the longitudinal direction.

各种环氧块体超薄截面收缩率的比较研究：从Spurr块体上进行切片会导致纵向收缩。

• 发表时间: 2012
• 期刊: Journal of Electron Microscopy Technology for Medicine and Biology
• 作者: 仁木智哉;間竜太郎;仁木朋子;西島隆明;Inoue,K.
• 通讯作者: Inoue,K.

In situlocalization of avenanthramide A and its biosynthetic enzyme in oat leaves infected with the crown rust fungus

燕麦菌胺 A 及其生物合成酶在感染冠锈菌的燕麦叶中的原位定位

• 发表时间: 2011
• 期刊: Puccinia coronataf . sp.Avenae, Physiological and Molecular Plant Pathology
• 作者: Uchihashi,K,. Nakayashiki,H.;Okamura,K.;Ishihara;A.;Tosa,Y. Park,P.;Mayama;S.
• 通讯作者: S.