Immunoelectron microscopy using aldehyde fixation with oxygen- bubbling for highly preserving protein in pathogen-infected anduninfected plant tissues

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A new chemical fixation method was developed successfully in this project. On fixing plants with glutaraldehyde together with oxygen bubbling, the higher preservation of glycoproteins was achieved, resulting in observing fine ultrastructure under electron microscope. The cell images involving membranes and matrix of organelles, cytoplasm and cell components were excellent. However, it was not yet apparent whether the bubbling fixation was good enough to the preservation of protein for immunoelectron microscopy or not.